PMID- 20185687
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20220317
IS  - 1522-1547 (Electronic)
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 298
IP  - 5
DP  - 2010 May
TI  - Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem 
      cells that form organoids in vitro.
PG  - G590-600
LID - 10.1152/ajpgi.00470.2009 [doi]
AB  - The inability to identify, isolate, and culture intestinal epithelial stem cells 
      (IESCs) has been prohibitive to the study and therapeutic utilization of these 
      cells. Using a Sox9(EGFP) mouse model, we demonstrate that Sox9(EGFP) 
      fluorescence signatures can be used to differentiate between and enrich for 
      progenitors (Sox9(EGFPsubLo)) and multipotent IESCs (Sox9(EGFPlo)). Sox9(EGFPlo) 
      cells generate "organoids" in a recently defined culture system that mimics the 
      native IESC niche. These organoids possess all four differentiated cell types of 
      the small intestine epithelium, demonstrating the multipotent capacity of 
      Sox9(EGFPlo) cells. Our results are consistent with the previously reported 
      observation that single IESCs generate cryptlike units without a detectable 
      mesenchymal cell component. A prospective search revealed that CD24 is expressed 
      in the Sox9(EGFPlo) population and marks IESCs that form organoids in culture. 
      CD24 represents the first cell surface marker that facilitates 
      fluorescence-activated cell sorting enrichment of IESCs with widely available 
      antibodies without requiring a specialized fluorescent reporter gene mouse model.
FAU - Gracz, Adam D
AU  - Gracz AD
AD  - Department of Medicine, University of North Carolina at Chapel Hill, USA.
FAU - Ramalingam, Sendhilnathan
AU  - Ramalingam S
FAU - Magness, Scott T
AU  - Magness ST
LA  - eng
GR  - P30 DK034987/DK/NIDDK NIH HHS/United States
GR  - P30 NS045892-04/NS/NINDS NIH HHS/United States
GR  - 1-K01-DK080181-01/DK/NIDDK NIH HHS/United States
GR  - P30 NS045892/NS/NINDS NIH HHS/United States
GR  - 5P30DK034987/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100225
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (CD24 Antigen)
RN  - 0 (SOX9 Transcription Factor)
SB  - IM
MH  - Animals
MH  - CD24 Antigen/*genetics
MH  - Cell Differentiation
MH  - Cell Line
MH  - Epithelial Cells/metabolism
MH  - Flow Cytometry
MH  - Intestine, Small/cytology/immunology/*metabolism
MH  - Mice
MH  - Organoids/*growth & development
MH  - SOX9 Transcription Factor/*biosynthesis
MH  - Stem Cells/cytology
PMC - PMC2867430
EDAT- 2010/02/27 06:00
MHDA- 2010/05/07 06:00
PMCR- 2011/05/01
CRDT- 2010/02/27 06:00
PHST- 2010/02/27 06:00 [entrez]
PHST- 2010/02/27 06:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - ajpgi.00470.2009 [pii]
AID - GI-00470-2009 [pii]
AID - 10.1152/ajpgi.00470.2009 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2010 May;298(5):G590-600. doi: 
      10.1152/ajpgi.00470.2009. Epub 2010 Feb 25.