PMID- 20215351
OWN - NLM
STAT- MEDLINE
DCOM- 20100409
LR  - 20190524
IS  - 1477-9129 (Electronic)
IS  - 0950-1991 (Linking)
VI  - 137
IP  - 7
DP  - 2010 Apr
TI  - Cell death-induced regeneration in wing imaginal discs requires JNK signalling.
PG  - 1169-79
LID - 10.1242/dev.045559 [doi]
AB  - Regeneration and tissue repair allow damaged or lost body parts to be replaced. 
      After injury or fragmentation of Drosophila imaginal discs, regeneration leads to 
      the development of normal adult structures. This process is likely to involve a 
      combination of cell rearrangement and compensatory proliferation. However, the 
      detailed mechanisms underlying these processes are poorly understood. We have 
      established a system to allow temporally restricted induction of cell death in 
      situ. Using Gal4/Gal80 and UAS-rpr constructs, targeted ablation of a region of 
      the disc could be performed and regeneration monitored without the requirement 
      for microsurgical manipulation. Using a ptc-Gal4 construct to drive rpr 
      expression in the wing disc resulted in a stripe of dead cells in the anterior 
      compartment flanking the anteroposterior boundary, whereas a sal-Gal4 driver 
      generated a dead domain that includes both anterior and posterior cells. Under 
      these conditions, regenerated tissues were derived from the damaged compartment, 
      suggesting that compartment restrictions are preserved during regeneration. Our 
      studies reveal that during regeneration the live cells bordering the domain in 
      which cell death was induced first display cytoskeletal reorganisation and 
      apical-to-basal closure of the epithelium. Then, proliferation begins locally in 
      the vicinity of the wound and later more extensively in the affected compartment. 
      Finally, we show that regeneration of genetically ablated tissue requires JNK 
      activity. During cell death-induced regeneration, the JNK pathway is activated at 
      the leading edges of healing tissue and not in the apoptotic cells, and is 
      required for the regulation of healing and regenerative growth.
FAU - Bergantinos, Cora
AU  - Bergantinos C
AD  - Departament de Genetica, Facultat de Biologia, and Institut de Biomedicina de la 
      Universitat de Barcelona IBUB, Universitat de Barcelona, Diagonal 645, 08028 
      Barcelona, Spain.
FAU - Corominas, Montserrat
AU  - Corominas M
FAU - Serras, Florenci
AU  - Serras F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Drosophila Proteins)
RN  - 0 (rpr protein, Drosophila)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Death/*physiology
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Cytoskeleton/metabolism/ultrastructure
MH  - Drosophila Proteins/genetics/metabolism
MH  - *Drosophila melanogaster/anatomy & histology/embryology/physiology
MH  - *Embryo, Nonmammalian/anatomy & histology/physiology
MH  - JNK Mitogen-Activated Protein Kinases/genetics/*metabolism
MH  - Mitosis/physiology
MH  - Regeneration/*physiology
MH  - Signal Transduction/*physiology
MH  - Wings, Animal/anatomy & histology/embryology/physiology
EDAT- 2010/03/11 06:00
MHDA- 2010/04/10 06:00
CRDT- 2010/03/11 06:00
PHST- 2010/03/11 06:00 [entrez]
PHST- 2010/03/11 06:00 [pubmed]
PHST- 2010/04/10 06:00 [medline]
AID - 137/7/1169 [pii]
AID - 10.1242/dev.045559 [doi]
PST - ppublish
SO  - Development. 2010 Apr;137(7):1169-79. doi: 10.1242/dev.045559.