PMID- 20226166
OWN - NLM
STAT- MEDLINE
DCOM- 20100504
LR  - 20220410
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 394
IP  - 3
DP  - 2010 Apr 9
TI  - MicroRNA-375 targets Hippo-signaling effector YAP in liver cancer and inhibits 
      tumor properties.
PG  - 623-7
LID - 10.1016/j.bbrc.2010.03.036 [doi]
AB  - Hepatocellular carcinoma (HCC) is a malignant form of liver cancer that ranks the 
      second leading cause of cancer-related deaths in China and many Asia regions. The 
      dismal outcome reflects the need for a better understanding of the 
      transcriptional control of oncogenic signaling pathway. Our recent findings have 
      identified yes-associated protein (YAP) is a potent oncogenic driver and 
      independent prognostic risk factor of HCC. The present study aims to elucidate 
      the transcriptional regulation of YAP targeted by microRNA (miRNA). miR-375 is a 
      putative target and was found significantly down-regulated in the tumor versus 
      adjacent non-tumor tissues of HCC patients (n=48). As determined by luciferase 
      reporter assay, we found ectopic expression of miR-375 could diminish the 
      transcriptional activity of YAP. Furthermore, immunoblotting revealed miR-375 
      suppressed endogenous YAP protein level. Functional assays showed that miR-375 
      was able to inhibit proliferation and invasion of HCC cells. CONCLUSION: miR-375 
      is an important regulator of YAP oncogene, implicating a potential therapeutic 
      role in HCC treatment.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Liu, Angela M
AU  - Liu AM
AD  - Department of Surgery, The University of Hong Kong, Queen Mary Hospital, 
      Pokfulam, Hong Kong Special Administrative Region, China.
FAU - Poon, Ronnie T P
AU  - Poon RT
FAU - Luk, John M
AU  - Luk JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100310
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (MIRN375 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (YY1AP1 protein, human)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Base Sequence
MH  - Carcinoma, Hepatocellular/*genetics/pathology/therapy
MH  - Cell Cycle Proteins
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Genes, Reporter
MH  - Humans
MH  - Liver Neoplasms/*genetics/pathology/therapy
MH  - Luciferases/genetics
MH  - Male
MH  - MicroRNAs/genetics/*metabolism
MH  - Neoplasm Invasiveness
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Transcription Factors/*genetics/metabolism
EDAT- 2010/03/17 06:00
MHDA- 2010/05/05 06:00
CRDT- 2010/03/16 06:00
PHST- 2010/03/02 00:00 [received]
PHST- 2010/03/05 00:00 [accepted]
PHST- 2010/03/16 06:00 [entrez]
PHST- 2010/03/17 06:00 [pubmed]
PHST- 2010/05/05 06:00 [medline]
AID - S0006-291X(10)00474-2 [pii]
AID - 10.1016/j.bbrc.2010.03.036 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Apr 9;394(3):623-7. doi: 
      10.1016/j.bbrc.2010.03.036. Epub 2010 Mar 10.