PMID- 20346707
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20230620
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 32
IP  - 4
DP  - 2010 Apr 23
TI  - T cell receptor "inside-out" pathway via signaling module SKAP1-RapL regulates T 
      cell motility and interactions in lymph nodes.
PG  - 541-56
LID - 10.1016/j.immuni.2010.03.007 [doi]
AB  - Although essential for T cell function, the identity of the T cell receptor 
      "inside-out" pathway for lymphocyte function-associated antigen 1 (LFA-1) 
      adhesion has proved elusive. Here, we define the "inside-out" pathway mediated by 
      N-terminal SKAP1 (SKAP-55) domain binding to the C-terminal SARAH domain of RapL. 
      TcR induced Rap1-RapL complex formation and LFA-1 binding failed to occur in 
      Skap1(-/-) primary T cells. SKAP1 generated a SKAP1-RapL-Rap1 complex that bound 
      to LFA-1, whereas a RapL mutation (L224A) that abrogated SKAP1 binding without 
      affecting MST1 disrupted component colocalization in vesicles as well as T 
      cell-dendritic cell (DC) conjugation. RapL expression also "slowed" T cell 
      motility in D011.10 transgenic T cells in lymph nodes (LNs), an effect reversed 
      by the L224A mutation with reduced dwell times between T cells and DCs. Overall, 
      our findings define a TCR "inside-out" pathway via N-SKAP1-C-RapL that regulates 
      T cell adhesion, motility, and arrest times with DCs in LNs.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Raab, Monika
AU  - Raab M
AD  - Cell Signaling Section, Department of Pathology, Tennis Court Road, University of 
      Cambridge, Cambridge UK, CB2 1Q.
FAU - Wang, Hongyan
AU  - Wang H
FAU - Lu, Yuning
AU  - Lu Y
FAU - Smith, Xin
AU  - Smith X
FAU - Wu, Zhonglin
AU  - Wu Z
FAU - Strebhardt, Klaus
AU  - Strebhardt K
FAU - Ladbury, John E
AU  - Ladbury JE
FAU - Rudd, Christopher E
AU  - Rudd CE
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
GR  - 092627/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100325
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 0 (Phosphoproteins)
RN  - 0 (RAPL protein, mouse)
RN  - 0 (RASSF5 protein, human)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (SKAP1 protein, human)
RN  - 0 (SKAP1 protein, mouse)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rap1 GTP-Binding Proteins)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Apoptosis Regulatory Proteins
MH  - Cell Adhesion
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology
MH  - Lymph Nodes/cytology/*immunology/metabolism
MH  - Lymphocyte Function-Associated Antigen-1/immunology/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Monomeric GTP-Binding Proteins/genetics/immunology/*metabolism
MH  - Mutation
MH  - Phosphoproteins/deficiency/genetics/*immunology/*metabolism
MH  - Protein Binding
MH  - Protein Transport
MH  - Receptors, Antigen, T-Cell/*immunology/metabolism
MH  - Signal Transduction
MH  - T-Lymphocytes/cytology/*immunology/metabolism
MH  - rap1 GTP-Binding Proteins/immunology/*metabolism
PMC - PMC3812847
MID - EMS54931
OID - NLM: EMS54931
EDAT- 2010/03/30 06:00
MHDA- 2010/05/07 06:00
PMCR- 2013/10/30
CRDT- 2010/03/30 06:00
PHST- 2009/05/03 00:00 [received]
PHST- 2009/11/05 00:00 [revised]
PHST- 2010/01/15 00:00 [accepted]
PHST- 2010/03/30 06:00 [entrez]
PHST- 2010/03/30 06:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PHST- 2013/10/30 00:00 [pmc-release]
AID - S1074-7613(10)00090-7 [pii]
AID - 10.1016/j.immuni.2010.03.007 [doi]
PST - ppublish
SO  - Immunity. 2010 Apr 23;32(4):541-56. doi: 10.1016/j.immuni.2010.03.007. Epub 2010 
      Mar 25.