PMID- 20386695
OWN - NLM
STAT- MEDLINE
DCOM- 20110616
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 5
IP  - 4
DP  - 2010 Apr 8
TI  - Identification of candidate growth promoting genes in ovarian cancer through 
      integrated copy number and expression analysis.
PG  - e9983
LID - 10.1371/journal.pone.0009983 [doi]
LID - e9983
AB  - Ovarian cancer is a disease characterised by complex genomic rearrangements but 
      the majority of the genes that are the target of these alterations remain 
      unidentified. Cataloguing these target genes will provide useful insights into 
      the disease etiology and may provide an opportunity to develop novel diagnostic 
      and therapeutic interventions. High resolution genome wide copy number and 
      matching expression data from 68 primary epithelial ovarian carcinomas of various 
      histotypes was integrated to identify genes in regions of most frequent 
      amplification with the strongest correlation with expression and copy number. 
      Regions on chromosomes 3, 7, 8, and 20 were most frequently increased in copy 
      number (> 40% of samples). Within these regions, 703/1370 (51%) unique gene 
      expression probesets were differentially expressed when samples with gain were 
      compared to samples without gain. 30% of these differentially expressed probesets 
      also showed a strong positive correlation (r > or =0.6) between expression and 
      copy number. We also identified 21 regions of high amplitude copy number gain, in 
      which 32 known protein coding genes showed a strong positive correlation between 
      expression and copy number. Overall, our data validates previously known ovarian 
      cancer genes, such as ERBB2, and also identified novel potential drivers such as 
      MYNN, PUF60 and TPX2.
FAU - Ramakrishna, Manasa
AU  - Ramakrishna M
AD  - VBCRC Cancer Genetics Laboratory, Peter MacCallum Cancer Centre, East Melbourne, 
      Victoria, Australia.
FAU - Williams, Louise H
AU  - Williams LH
FAU - Boyle, Samantha E
AU  - Boyle SE
FAU - Bearfoot, Jennifer L
AU  - Bearfoot JL
FAU - Sridhar, Anita
AU  - Sridhar A
FAU - Speed, Terence P
AU  - Speed TP
FAU - Gorringe, Kylie L
AU  - Gorringe KL
FAU - Campbell, Ian G
AU  - Campbell IG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100408
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (MYNN protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (TPX2 protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
EIN - PLoS One. 2011;6(7). doi:10.1371/annotation/4056b510-e92d-4472-871f-2cf1f6834689
MH  - Cell Cycle Proteins/genetics
MH  - Chromosomes
MH  - DNA-Binding Proteins
MH  - Female
MH  - *Gene Dosage
MH  - *Gene Expression Profiling
MH  - Genes, erbB-2
MH  - Humans
MH  - Kruppel-Like Transcription Factors/genetics
MH  - Microtubule-Associated Proteins/genetics
MH  - Nuclear Proteins/genetics
MH  - Ovarian Neoplasms/*genetics
MH  - Transcription Factors
PMC - PMC2851616
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2010/04/14 06:00
MHDA- 2011/06/17 06:00
PMCR- 2010/04/08
CRDT- 2010/04/14 06:00
PHST- 2010/01/20 00:00 [received]
PHST- 2010/03/07 00:00 [accepted]
PHST- 2010/04/14 06:00 [entrez]
PHST- 2010/04/14 06:00 [pubmed]
PHST- 2011/06/17 06:00 [medline]
PHST- 2010/04/08 00:00 [pmc-release]
AID - 10-PONE-RA-15644R1 [pii]
AID - 10.1371/journal.pone.0009983 [doi]
PST - epublish
SO  - PLoS One. 2010 Apr 8;5(4):e9983. doi: 10.1371/journal.pone.0009983.