PMID- 20418863
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20220331
IS  - 1476-4660 (Electronic)
IS  - 1476-1122 (Print)
IS  - 1476-1122 (Linking)
VI  - 9
IP  - 6
DP  - 2010 Jun
TI  - Harnessing traction-mediated manipulation of the cell/matrix interface to control 
      stem-cell fate.
PG  - 518-26
LID - 10.1038/nmat2732 [doi]
AB  - Stem cells sense and respond to the mechanical properties of the extracellular 
      matrix. However, both the extent to which extracellular-matrix mechanics affect 
      stem-cell fate in three-dimensional microenvironments and the underlying 
      biophysical mechanisms are unclear. We demonstrate that the commitment of 
      mesenchymal stem-cell populations changes in response to the rigidity of 
      three-dimensional microenvironments, with osteogenesis occurring predominantly at 
      11-30 kPa. In contrast to previous two-dimensional work, however, cell fate was 
      not correlated with morphology. Instead, matrix stiffness regulated integrin 
      binding as well as reorganization of adhesion ligands on the nanoscale, both of 
      which were traction dependent and correlated with osteogenic commitment of 
      mesenchymal stem-cell populations. These findings suggest that cells interpret 
      changes in the physical properties of adhesion substrates as changes in 
      adhesion-ligand presentation, and that cells themselves can be harnessed as tools 
      to mechanically process materials into structures that feed back to manipulate 
      their fate.
FAU - Huebsch, Nathaniel
AU  - Huebsch N
AD  - School of Engineering and Applied Sciences, Harvard University, Cambridge, 
      Massachusetts 02138, USA.
FAU - Arany, Praveen R
AU  - Arany PR
FAU - Mao, Angelo S
AU  - Mao AS
FAU - Shvartsman, Dmitry
AU  - Shvartsman D
FAU - Ali, Omar A
AU  - Ali OA
FAU - Bencherif, Sidi A
AU  - Bencherif SA
FAU - Rivera-Feliciano, Jose
AU  - Rivera-Feliciano J
FAU - Mooney, David J
AU  - Mooney DJ
LA  - eng
GR  - R37 DE013033/DE/NIDCR NIH HHS/United States
GR  - R37 DE013033-13/DE/NIDCR NIH HHS/United States
GR  - TL1 EB008540/EB/NIBIB NIH HHS/United States
GR  - 1TL1EB008540-01/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100425
PL  - England
TA  - Nat Mater
JT  - Nature materials
JID - 101155473
RN  - 0 (Alginates)
RN  - 0 (Hydrogels)
RN  - 0 (Integrins)
SB  - IM
CIN - Cell Stem Cell. 2010 Jun 4;6(6):499-501. PMID: 20569684
MH  - Alginates
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Biophysics
MH  - Cell Culture Techniques
MH  - Cell Transplantation/physiology
MH  - Extracellular Matrix/*physiology/ultrastructure
MH  - Humans
MH  - Hydrogels
MH  - Integrins/physiology
MH  - Mesenchymal Stem Cells/*cytology/*physiology
MH  - Microscopy/methods
MH  - Osteogenesis/physiology
MH  - Stem Cells/*cytology/*physiology
PMC - PMC2919753
MID - NIHMS183313
EDAT- 2010/04/27 06:00
MHDA- 2010/07/08 06:00
PMCR- 2010/12/01
CRDT- 2010/04/27 06:00
PHST- 2009/09/24 00:00 [received]
PHST- 2010/02/26 00:00 [accepted]
PHST- 2010/04/27 06:00 [entrez]
PHST- 2010/04/27 06:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PHST- 2010/12/01 00:00 [pmc-release]
AID - nmat2732 [pii]
AID - 10.1038/nmat2732 [doi]
PST - ppublish
SO  - Nat Mater. 2010 Jun;9(6):518-26. doi: 10.1038/nmat2732. Epub 2010 Apr 25.