PMID- 20434990
OWN - NLM
STAT- MEDLINE
DCOM- 20100507
LR  - 20220530
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 141
IP  - 3
DP  - 2010 Apr 30
TI  - The Q system: a repressible binary system for transgene expression, lineage 
      tracing, and mosaic analysis.
PG  - 536-48
LID - 10.1016/j.cell.2010.02.025 [doi]
AB  - We describe a new repressible binary expression system based on the regulatory 
      genes from the Neurospora qa gene cluster. This "Q system" offers attractive 
      features for transgene expression in Drosophila and mammalian cells: low basal 
      expression in the absence of the transcriptional activator QF, high QF-induced 
      expression, and QF repression by its repressor QS. Additionally, feeding flies 
      quinic acid can relieve QS repression. The Q system offers many applications, 
      including (1) intersectional "logic gates" with the GAL4 system for manipulating 
      transgene expression patterns, (2) GAL4-independent MARCM analysis, and (3) 
      coupled MARCM analysis to independently visualize and genetically manipulate 
      siblings from any cell division. We demonstrate the utility of the Q system in 
      determining cell division patterns of a neuronal lineage and gene function in 
      cell growth and proliferation, and in dissecting neurons responsible for 
      olfactory attraction. The Q system can be expanded to other uses in Drosophila 
      and to any organism conducive to transgenesis.
CI  - 2010 Elsevier Inc. All rights reserved.
FAU - Potter, Christopher J
AU  - Potter CJ
AD  - Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
FAU - Tasic, Bosiljka
AU  - Tasic B
FAU - Russler, Emilie V
AU  - Russler EV
FAU - Liang, Liang
AU  - Liang L
FAU - Luo, Liqun
AU  - Luo L
LA  - eng
SI  - GENBANK/HM068612
SI  - GENBANK/HM068613
SI  - GENBANK/HM068614
GR  - R37 NS041044/NS/NINDS NIH HHS/United States
GR  - R01 DC005982/DC/NIDCD NIH HHS/United States
GR  - R01 NS050835/NS/NINDS NIH HHS/United States
GR  - R01 DC005982-09/DC/NIDCD NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R37 NS041044-11/NS/NINDS NIH HHS/United States
GR  - R01 NS050835-07/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
SB  - IM
MH  - Animals
MH  - Cell Lineage
MH  - *Cytological Techniques
MH  - Drosophila/cytology
MH  - Female
MH  - *Genetic Techniques
MH  - HeLa Cells
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Neurospora crassa/genetics
MH  - Plasmids/chemistry/genetics
MH  - Transgenes
PMC - PMC2883883
MID - NIHMS202722
EDAT- 2010/05/04 06:00
MHDA- 2010/05/08 06:00
PMCR- 2010/10/30
CRDT- 2010/05/04 06:00
PHST- 2009/08/04 00:00 [received]
PHST- 2010/01/07 00:00 [revised]
PHST- 2010/02/16 00:00 [accepted]
PHST- 2010/05/04 06:00 [entrez]
PHST- 2010/05/04 06:00 [pubmed]
PHST- 2010/05/08 06:00 [medline]
PHST- 2010/10/30 00:00 [pmc-release]
AID - S0092-8674(10)00178-9 [pii]
AID - 10.1016/j.cell.2010.02.025 [doi]
PST - ppublish
SO  - Cell. 2010 Apr 30;141(3):536-48. doi: 10.1016/j.cell.2010.02.025.