PMID- 20436478 OWN - NLM STAT- MEDLINE DCOM- 20100614 LR - 20220309 IS - 1546-1726 (Electronic) IS - 1097-6256 (Linking) VI - 13 IP - 6 DP - 2010 Jun TI - OSVZ progenitors of human and ferret neocortex are epithelial-like and expand by integrin signaling. PG - 690-9 LID - 10.1038/nn.2553 [doi] AB - A major cause of the cerebral cortex expansion that occurred during evolution is the increase in subventricular zone (SVZ) progenitors. We found that progenitors in the outer SVZ (OSVZ) of developing human neocortex retain features of radial glia, in contrast to rodent SVZ progenitors, which have limited proliferation potential. Although delaminating from apical adherens junctions, OSVZ progenitors maintained a basal process contacting the basal lamina, a canonical epithelial property. OSVZ progenitor divisions resulted in asymmetric inheritance of their basal process. Notably, OSVZ progenitors are also found in the ferret, a gyrencephalic nonprimate. Functional disruption of integrins, expressed on the basal process of ferret OSVZ progenitors, markedly decreased the OSVZ progenitor population size, but not that of other, process-lacking SVZ progenitors, in slice cultures of ferret neocortex. Our findings suggest that maintenance of this epithelial property allows integrin-mediated, repeated asymmetric divisions of OSVZ progenitors, providing a basis for neocortical expansion. FAU - Fietz, Simone A AU - Fietz SA AD - Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. FAU - Kelava, Iva AU - Kelava I FAU - Vogt, Johannes AU - Vogt J FAU - Wilsch-Brauninger, Michaela AU - Wilsch-Brauninger M FAU - Stenzel, Denise AU - Stenzel D FAU - Fish, Jennifer L AU - Fish JL FAU - Corbeil, Denis AU - Corbeil D FAU - Riehn, Axel AU - Riehn A FAU - Distler, Wolfgang AU - Distler W FAU - Nitsch, Robert AU - Nitsch R FAU - Huttner, Wieland B AU - Huttner WB LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20100502 PL - United States TA - Nat Neurosci JT - Nature neuroscience JID - 9809671 RN - 0 (Integrin beta3) RN - 0 (Integrins) RN - 0 (Paired Box Transcription Factors) SB - IM CIN - Nat Neurosci. 2010 Jun;13(6):656-7. PMID: 20498684 MH - Animals MH - Cell Count MH - Cell Division/physiology MH - Centrosome/physiology/ultrastructure MH - Epithelial Cells/physiology MH - Ferrets MH - Humans MH - Immunohistochemistry MH - In Situ Hybridization MH - In Vitro Techniques MH - Integrin beta3/metabolism MH - Integrins/*metabolism MH - Microscopy, Electron MH - Neocortex/*embryology/*physiology/ultrastructure MH - Neuroglia/physiology/ultrastructure MH - Paired Box Transcription Factors/metabolism MH - Species Specificity MH - Stem Cell Niche/*embryology/*physiology/ultrastructure MH - Stem Cells/*physiology/ultrastructure EDAT- 2010/05/04 06:00 MHDA- 2010/06/15 06:00 CRDT- 2010/05/04 06:00 PHST- 2010/02/09 00:00 [received] PHST- 2010/04/19 00:00 [accepted] PHST- 2010/05/04 06:00 [entrez] PHST- 2010/05/04 06:00 [pubmed] PHST- 2010/06/15 06:00 [medline] AID - nn.2553 [pii] AID - 10.1038/nn.2553 [doi] PST - ppublish SO - Nat Neurosci. 2010 Jun;13(6):690-9. doi: 10.1038/nn.2553. Epub 2010 May 2.