PMID- 20466877
OWN - NLM
STAT- MEDLINE
DCOM- 20100921
LR  - 20211203
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 24
IP  - 9
DP  - 2010 Sep
TI  - TAZ as a novel enhancer of MyoD-mediated myogenic differentiation.
PG  - 3310-20
LID - 10.1096/fj.09-151324 [doi]
AB  - Myoblast differentiation is indispensable for skeletal muscle formation and is 
      governed by the precisely coordinated regulation of a series of transcription 
      factors, including MyoD and myogenin, and transcriptional coregulators. TAZ 
      (transcriptional coactivator with PDZ-binding motif) has been characterized as a 
      modulator of mesenchymal stem cell differentiation into osteoblasts and 
      adipocytes through its regulation of lineage-specific master transcription 
      factors. In this study, we investigated whether TAZ affects myoblast 
      differentiation, which is one of the differentiated lineages of mesenchymal stem 
      cells. Ectopic overexpression of TAZ in myoblasts increases myogenic gene 
      expression in a MyoD-dependent manner and hastens myofiber formation, whereas TAZ 
      knockdown delays myogenic differentiation. In addition, enforced coexpression of 
      TAZ and MyoD in fibroblasts accelerates MyoD-induced myogenic differentiation. 
      TAZ physically interacts with MyoD through the WW domain and activates 
      MyoD-dependent gene transcription. TAZ additionally enhances the interaction of 
      MyoD with the myogenin gene promoter. These results strongly suggest that TAZ 
      functions as a novel transcriptional modulator of myogenic differentiation by 
      promoting MyoD-mediated myogenic gene expression.
FAU - Jeong, Hana
AU  - Jeong H
AD  - College of Pharmacy and Division of Life and Pharmaceutical Sciences, Ewha 
      Woman's University, Science Bldg C206, 11-1 Daehyun-Dong, Sudaemun-Ku, Seoul 
      120-750, Korea.
FAU - Bae, Sujung
AU  - Bae S
FAU - An, Su Yeon
AU  - An SY
FAU - Byun, Mi Ran
AU  - Byun MR
FAU - Hwang, Jun-Ha
AU  - Hwang JH
FAU - Yaffe, Michael B
AU  - Yaffe MB
FAU - Hong, Jeong-Ho
AU  - Hong JH
FAU - Hwang, Eun Sook
AU  - Hwang ES
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100513
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (MyoD Protein)
RN  - 0 (Myogenin)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.- (tafazzin protein, mouse)
SB  - IM
MH  - Acyltransferases
MH  - Animals
MH  - Cell Differentiation/genetics/*physiology
MH  - Cell Line
MH  - Chromatin Immunoprecipitation
MH  - Electrophoretic Mobility Shift Assay
MH  - Fluorescent Antibody Technique
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Immunoprecipitation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MyoD Protein/genetics/*metabolism
MH  - Myoblasts/cytology/metabolism
MH  - Myogenin/genetics/metabolism
MH  - Polymerase Chain Reaction
MH  - Transcription Factors/genetics/*metabolism
EDAT- 2010/05/15 06:00
MHDA- 2010/09/23 06:00
CRDT- 2010/05/15 06:00
PHST- 2010/05/15 06:00 [entrez]
PHST- 2010/05/15 06:00 [pubmed]
PHST- 2010/09/23 06:00 [medline]
AID - fj.09-151324 [pii]
AID - 10.1096/fj.09-151324 [doi]
PST - ppublish
SO  - FASEB J. 2010 Sep;24(9):3310-20. doi: 10.1096/fj.09-151324. Epub 2010 May 13.