PMID- 20468047
OWN - NLM
STAT- MEDLINE
DCOM- 20100826
LR  - 20211020
IS  - 1098-1136 (Electronic)
IS  - 0894-1491 (Linking)
VI  - 58
IP  - 9
DP  - 2010 Jul
TI  - Glial progenitors in the brainstem give rise to malignant gliomas by 
      platelet-derived growth factor stimulation.
PG  - 1050-65
LID - 10.1002/glia.20986 [doi]
AB  - Glial progenitors in the white matter and the subventricular zone are the major 
      population of cycling cells in the postnatal central nervous system, and thought 
      to be candidates for glioma-initiating cells. However, less is known about the 
      dividing cell populations in the brainstem than those in the cerebrum, leading to 
      the lag of basic understanding of brainstem gliomas. We herein demonstrate much 
      fewer cycling glial progenitors exist in the brainstem than in the cerebrum. We 
      also show that infecting brainstem glial progenitors with PDGFB-green fluorescent 
      protein (GFP)-expressing retrovirus induced tumors that closely resembled human 
      malignant gliomas. Of note, brainstem tumors grew more slowly than cerebral 
      tumors induced by the same retrovirus, and >80% tumor cells in the brainstem 
      consisted of GFP-positive, infected progenitors while GFP-positive cells in the 
      cerebral tumors were <20%. These indicate that cerebral tumors progressed rapidly 
      by recruiting resident progenitors via paracrine mechanism whereas brainstem 
      tumors grew more slowly by clonal expansion of the infected population. The 
      cerebral and brainstem glial progenitors similarly showed reversible 
      dedifferentiation upon PDGF stimulation in vitro and did not show the intrinsic 
      difference in terms of the responsiveness to PDGF. We therefore suggest that 
      slower, monoclonal progression pattern of the brainstem tumors is at least partly 
      due to the environmental factors including the cell density of the glial 
      progenitors. Together, these findings are the first implications regarding the 
      cell-of-origin and the gliomagenesis in the brainstem.
CI  - Copyright 2010 Wiley-Liss, Inc.
FAU - Masui, Kenta
AU  - Masui K
AD  - Department of Neuropathology, Neurological Institute, Graduate School of Medical 
      Sciences, Kyushu University, Fukuoka, Japan.
FAU - Suzuki, Satoshi O
AU  - Suzuki SO
FAU - Torisu, Rina
AU  - Torisu R
FAU - Goldman, James E
AU  - Goldman JE
FAU - Canoll, Peter
AU  - Canoll P
FAU - Iwaki, Toru
AU  - Iwaki T
LA  - eng
GR  - R01 NS066955/NS/NINDS NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Brain Neoplasms/physiopathology
MH  - Brain Stem/pathology/*physiopathology
MH  - Brain Stem Neoplasms/pathology/*physiopathology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cerebellum/physiology
MH  - Child
MH  - Female
MH  - Genes, sis
MH  - Genetic Vectors
MH  - Glioma/pathology/*physiopathology
MH  - Green Fluorescent Proteins/genetics
MH  - Humans
MH  - Male
MH  - Neuroglia/*physiology
MH  - Platelet-Derived Growth Factor/genetics/*metabolism
MH  - Rats
MH  - Retroviridae
MH  - Stem Cells/*physiology
EDAT- 2010/05/15 06:00
MHDA- 2010/08/27 06:00
CRDT- 2010/05/15 06:00
PHST- 2010/05/15 06:00 [entrez]
PHST- 2010/05/15 06:00 [pubmed]
PHST- 2010/08/27 06:00 [medline]
AID - 10.1002/glia.20986 [doi]
PST - ppublish
SO  - Glia. 2010 Jul;58(9):1050-65. doi: 10.1002/glia.20986.