PMID- 20479129
OWN - NLM
STAT- MEDLINE
DCOM- 20100714
LR  - 20211020
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 30
IP  - 14
DP  - 2010 Jul
TI  - Ric-8A and Gi alpha recruit LGN, NuMA, and dynein to the cell cortex to help 
      orient the mitotic spindle.
PG  - 3519-30
LID - 10.1128/MCB.00394-10 [doi]
AB  - In model organisms, resistance to inhibitors of cholinesterase 8 (Ric-8), a G 
      protein alpha (G alpha) subunit guanine nucleotide exchange factor (GEF), 
      functions to orient mitotic spindles during asymmetric cell divisions; however, 
      whether Ric-8A has any role in mammalian cell division is unknown. We show here 
      that Ric-8A and G alpha(i) function to orient the metaphase mitotic spindle of 
      mammalian adherent cells. During mitosis, Ric-8A localized at the cell cortex, 
      spindle poles, centromeres, central spindle, and midbody. Pertussis toxin proved 
      to be a useful tool in these studies since it blocked the binding of Ric-8A to G 
      alpha(i), thus preventing its GEF activity for G alpha(i). Linking Ric-8A 
      signaling to mammalian cell division, treatment of cells with pertussis toxin, 
      reduction of Ric-8A expression, or decreased G alpha(i) expression similarly 
      affected metaphase cells. Each treatment impaired the localization of LGN 
      (GSPM2), NuMA (microtubule binding nuclear mitotic apparatus protein), and dynein 
      at the metaphase cell cortex and disturbed integrin-dependent mitotic spindle 
      orientation. Live cell imaging of HeLa cells expressing green fluorescent 
      protein-tubulin also revealed that reduced Ric-8A expression prolonged mitosis, 
      caused occasional mitotic arrest, and decreased mitotic spindle movements. These 
      data indicate that Ric-8A signaling leads to assembly of a cortical signaling 
      complex that functions to orient the mitotic spindle.
FAU - Woodard, Geoffrey E
AU  - Woodard GE
AD  - B-Cell Molecular Immunology Section, Laboratory of Immunoregulation, National 
      Institute of Allergy and Infectious Disease, 10 Center Drive, MSC 1888, Bethesda, 
      MD 20892-1752, USA.
FAU - Huang, Ning-Na
AU  - Huang NN
FAU - Cho, Hyeseon
AU  - Cho H
FAU - Miki, Toru
AU  - Miki T
FAU - Tall, Gregory G
AU  - Tall GG
FAU - Kehrl, John H
AU  - Kehrl JH
LA  - eng
GR  - R01 GM088242/GM/NIGMS NIH HHS/United States
GR  - ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20100517
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (GPSM2 protein, human)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (NUMA1 protein, human)
RN  - 0 (Nuclear Matrix-Associated Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 3.6.4.2 (Dyneins)
RN  - EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gi-Go)
SB  - IM
MH  - Animals
MH  - Antigens, Nuclear/*metabolism
MH  - Base Sequence
MH  - Cell Cycle Proteins
MH  - Cell Division/physiology
MH  - Cell Line
MH  - Dogs
MH  - Dyneins/*metabolism
MH  - GTP-Binding Protein alpha Subunits, Gi-Go/antagonists & 
      inhibitors/genetics/*metabolism
MH  - Guanine Nucleotide Exchange Factors/antagonists & inhibitors/genetics/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Interphase/physiology
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Metaphase/physiology
MH  - Nuclear Matrix-Associated Proteins/*metabolism
MH  - Pertussis Toxin/pharmacology
MH  - Protein Binding/drug effects
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction
MH  - Spindle Apparatus/drug effects/*metabolism
PMC - PMC2897540
EDAT- 2010/05/19 06:00
MHDA- 2010/07/16 06:00
PMCR- 2011/01/01
CRDT- 2010/05/19 06:00
PHST- 2010/05/19 06:00 [entrez]
PHST- 2010/05/19 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - MCB.00394-10 [pii]
AID - 0394-10 [pii]
AID - 10.1128/MCB.00394-10 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2010 Jul;30(14):3519-30. doi: 10.1128/MCB.00394-10. Epub 2010 May 
      17.