PMID- 20495069
OWN - NLM
STAT- MEDLINE
DCOM- 20100618
LR  - 20211020
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 184
IP  - 12
DP  - 2010 Jun 15
TI  - CD8+ cell depletion accelerates HIV-1 immunopathology in humanized mice.
PG  - 7082-91
LID - 10.4049/jimmunol.1000438 [doi]
AB  - Stable engraftment of human lymphoid tissue in NOD/scid-IL-2Rgammacnull mice 
      after CD34+ hematopoietic stem cell reconstitution permits the evaluation of 
      ongoing HIV-1 infection for weeks to months. We demonstrate that HIV-1-infected 
      rodents develop virus-specific cellular immune responses. CD8+ cell depletion, 2 
      or 5-7 wk after viral infection, resulted in a significant increase of HIV-1 
      load, robust immune cell activation, and cytopathology in lymphoid tissues but 
      preserved CD4/CD8 double-positive thymic T cell pools. Human CD8+ cells 
      reappeared in circulation as early as 2-3 wk. These data support a role of CD8+ 
      cells in viral surveillance and the relevance of this humanized mouse model for 
      the studies of HIV-1 pathobiology and virus-specific immunity.
FAU - Gorantla, Santhi
AU  - Gorantla S
AD  - Department of Pharmacology and Experimental Neuroscience, University of Nebraska 
      Medical Center, Omaha, NE 68198, USA.
FAU - Makarov, Edward
AU  - Makarov E
FAU - Finke-Dwyer, Jennifer
AU  - Finke-Dwyer J
FAU - Gebhart, Catherine L
AU  - Gebhart CL
FAU - Domm, William
AU  - Domm W
FAU - Dewhurst, Stephen
AU  - Dewhurst S
FAU - Gendelman, Howard E
AU  - Gendelman HE
FAU - Poluektova, Larisa Y
AU  - Poluektova LY
LA  - eng
GR  - R01 NS036126/NS/NINDS NIH HHS/United States
GR  - P01 NS043985-01A1/NS/NINDS NIH HHS/United States
GR  - P01 NS043985/NS/NINDS NIH HHS/United States
GR  - P01 NS043985-08/NS/NINDS NIH HHS/United States
GR  - R21NS060642-01/NS/NINDS NIH HHS/United States
GR  - 1 P01 NS043985-01/NS/NINDS NIH HHS/United States
GR  - R21 NS060642-02/NS/NINDS NIH HHS/United States
GR  - P01 NS043985-07/NS/NINDS NIH HHS/United States
GR  - P01 MH064570-049001/MH/NIMH NIH HHS/United States
GR  - P20 RR15635/RR/NCRR NIH HHS/United States
GR  - R21AI074351/AI/NIAID NIH HHS/United States
GR  - P01 MH064570/MH/NIMH NIH HHS/United States
GR  - 5 P01 MH64570-03/MH/NIMH NIH HHS/United States
GR  - R37 NS036126/NS/NINDS NIH HHS/United States
GR  - R21 NS060642/NS/NINDS NIH HHS/United States
GR  - R37 NS036126-11/NS/NINDS NIH HHS/United States
GR  - P20 RR015635/RR/NCRR NIH HHS/United States
GR  - R37 NS036126-10/NS/NINDS NIH HHS/United States
GR  - 2R37 NS36126/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100521
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Receptors, Interleukin-2)
SB  - IM
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cell Separation
MH  - Cord Blood Stem Cell Transplantation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - HIV Infections/*immunology
MH  - HIV-1/*immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, Knockout
MH  - Mice, SCID
MH  - Receptors, Interleukin-2/deficiency/genetics
PMC - PMC3008566
MID - NIHMS201497
EDAT- 2010/05/25 06:00
MHDA- 2010/06/19 06:00
PMCR- 2010/12/22
CRDT- 2010/05/25 06:00
PHST- 2010/05/25 06:00 [entrez]
PHST- 2010/05/25 06:00 [pubmed]
PHST- 2010/06/19 06:00 [medline]
PHST- 2010/12/22 00:00 [pmc-release]
AID - jimmunol.1000438 [pii]
AID - 10.4049/jimmunol.1000438 [doi]
PST - ppublish
SO  - J Immunol. 2010 Jun 15;184(12):7082-91. doi: 10.4049/jimmunol.1000438. Epub 2010 
      May 21.