PMID- 20504961
OWN - NLM
STAT- MEDLINE
DCOM- 20100622
LR  - 20240214
IS  - 1477-9129 (Electronic)
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 137
IP  - 13
DP  - 2010 Jul
TI  - Specific roles of Target of rapamycin in the control of stem cells and their 
      progeny in the Drosophila ovary.
PG  - 2117-26
LID - 10.1242/dev.050351 [doi]
AB  - Stem cells depend on intrinsic and local factors to maintain their identity and 
      activity, but they also sense and respond to changing external conditions. We 
      previously showed that germline stem cells (GSCs) and follicle stem cells (FSCs) 
      in the Drosophila ovary respond to diet via insulin signals. Insulin signals 
      directly modulate the GSC cell cycle at the G2 phase, but additional unknown 
      dietary mediators control both G1 and G2. Target of rapamycin, or TOR, is part of 
      a highly conserved nutrient-sensing pathway affecting growth, proliferation, 
      survival and fertility. Here, we show that optimal TOR activity maintains GSCs 
      but does not play a major role in FSC maintenance, suggesting differential 
      regulation of GSCs versus FSCs. TOR promotes GSC proliferation via G2 but 
      independently of insulin signaling, and TOR is required for the proliferation, 
      growth and survival of differentiating germ cells. We also report that TOR 
      controls the proliferation of FSCs but not of their differentiating progeny. 
      Instead, TOR controls follicle cell number by promoting survival, independently 
      of either the apoptotic or autophagic pathways. These results uncover specific 
      TOR functions in the control of stem cells versus their differentiating progeny, 
      and reveal parallels between Drosophila and mammalian follicle growth.
FAU - LaFever, Leesa
AU  - LaFever L
AD  - Department of Cell and Developmental Biology, Vanderbilt University Medical 
      Center, Nashville, TN 37232, USA.
FAU - Feoktistov, Alexander
AU  - Feoktistov A
FAU - Hsu, Hwei-Jan
AU  - Hsu HJ
FAU - Drummond-Barbosa, Daniela
AU  - Drummond-Barbosa D
LA  - eng
GR  - R01 GM069875/GM/NIGMS NIH HHS/United States
GR  - T32 HD007043/HD/NICHD NIH HHS/United States
GR  - T32 HD007502/HD/NICHD NIH HHS/United States
GR  - T32HD007043/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100526
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Drosophila Proteins)
RN  - 0 (Insulin)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (target of rapamycin protein, Drosophila)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
EIN - Development. 2010 Jul;137(14):2451
MH  - Animals
MH  - Cell Proliferation
MH  - Drosophila Proteins/*metabolism
MH  - Drosophila melanogaster/metabolism
MH  - Female
MH  - Insulin/metabolism
MH  - Ovary/cytology
MH  - Protein Kinases/*metabolism
MH  - Signal Transduction
MH  - Stem Cells/*metabolism
MH  - TOR Serine-Threonine Kinases
PMC - PMC2882131
EDAT- 2010/05/28 06:00
MHDA- 2010/06/23 06:00
PMCR- 2011/07/01
CRDT- 2010/05/28 06:00
PHST- 2010/05/28 06:00 [entrez]
PHST- 2010/05/28 06:00 [pubmed]
PHST- 2010/06/23 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - dev.050351 [pii]
AID - 10.1242/dev.050351 [doi]
PST - ppublish
SO  - Development. 2010 Jul;137(13):2117-26. doi: 10.1242/dev.050351. Epub 2010 May 26.