PMID- 20553005
OWN - NLM
STAT- MEDLINE
DCOM- 20101207
LR  - 20171116
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Linking)
VI  - 4
IP  - 7
DP  - 2010 Jul 27
TI  - Time evolution of the nanoparticle protein corona.
PG  - 3623-32
LID - 10.1021/nn901372t [doi]
AB  - In this work, we explore the formation of the protein corona after exposure of 
      metallic Au nanoparticles (NPs), with sizes ranging from 4 to 40 nm, to cell 
      culture media containing 10% of fetal bovine serum. Under in vitro cell culture 
      conditions, zeta potential measurements, UV-vis spectroscopy, dynamic light 
      scattering and transmission electron microscope analysis were used to monitor the 
      time evolution of the inorganic NP-protein corona formation and to characterize 
      the stability of the NPs and their surface state at every stage of the 
      experiment. As expected, the red-shift of the surface plasmon resonance peak, as 
      well as the drop of surface charge and the increase of the hydrodynamic diameter 
      indicated the conjugation of proteins to NPs. Remarkably, an evolution from a 
      loosely attached toward an irreversible attached protein corona over time was 
      observed. Mass spectrometry of the digested protein corona revealed albumin as 
      the most abundant component which suggests an improved biocompatibility.
FAU - Casals, Eudald
AU  - Casals E
AD  - Institut Catala de Nanotecnologia, Bellaterra, Barcelona, Spain.
FAU - Pfaller, Tobias
AU  - Pfaller T
FAU - Duschl, Albert
AU  - Duschl A
FAU - Oostingh, Gertie Janneke
AU  - Oostingh GJ
FAU - Puntes, Victor
AU  - Puntes V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (11-mercaptoundecanoic acid)
RN  - 0 (Citrates)
RN  - 0 (Fatty Acids)
RN  - 0 (Proteins)
RN  - 0 (Sulfhydryl Compounds)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Citrates/chemistry
MH  - Fatty Acids/chemistry
MH  - Gold/*chemistry
MH  - Metal Nanoparticles/*chemistry
MH  - Particle Size
MH  - Proteins/*chemistry
MH  - Serum Albumin, Bovine/chemistry
MH  - Static Electricity
MH  - Sulfhydryl Compounds/chemistry
MH  - Time Factors
EDAT- 2010/06/18 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/06/18 06:00
PHST- 2010/06/18 06:00 [entrez]
PHST- 2010/06/18 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1021/nn901372t [doi]
PST - ppublish
SO  - ACS Nano. 2010 Jul 27;4(7):3623-32. doi: 10.1021/nn901372t.