PMID- 20616235
OWN - NLM
STAT- MEDLINE
DCOM- 20100820
LR  - 20240105
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 329
IP  - 5992
DP  - 2010 Aug 6
TI  - Long noncoding RNA as modular scaffold of histone modification complexes.
PG  - 689-93
LID - 10.1126/science.1192002 [doi]
AB  - Long intergenic noncoding RNAs (lincRNAs) regulate chromatin states and 
      epigenetic inheritance. Here, we show that the lincRNA HOTAIR serves as a 
      scaffold for at least two distinct histone modification complexes. A 5' domain of 
      HOTAIR binds polycomb repressive complex 2 (PRC2), whereas a 3' domain of HOTAIR 
      binds the LSD1/CoREST/REST complex. The ability to tether two distinct complexes 
      enables RNA-mediated assembly of PRC2 and LSD1 and coordinates targeting of PRC2 
      and LSD1 to chromatin for coupled histone H3 lysine 27 methylation and lysine 4 
      demethylation. Our results suggest that lincRNAs may serve as scaffolds by 
      providing binding surfaces to assemble select histone modification enzymes, 
      thereby specifying the pattern of histone modifications on target genes.
FAU - Tsai, Miao-Chih
AU  - Tsai MC
AD  - Howard Hughes Medical Institute and Program in Epithelial Biology, Stanford 
      University School of Medicine, Stanford, CA 94305, USA.
FAU - Manor, Ohad
AU  - Manor O
FAU - Wan, Yue
AU  - Wan Y
FAU - Mosammaparast, Nima
AU  - Mosammaparast N
FAU - Wang, Jordon K
AU  - Wang JK
FAU - Lan, Fei
AU  - Lan F
FAU - Shi, Yang
AU  - Shi Y
FAU - Segal, Eran
AU  - Segal E
FAU - Chang, Howard Y
AU  - Chang HY
LA  - eng
SI  - GEO/GSE22345
GR  - R01 CA119176/CA/NCI NIH HHS/United States
GR  - R01 HG004361/HG/NHGRI NIH HHS/United States
GR  - R01 CA118487/CA/NCI NIH HHS/United States
GR  - R01-HG004361/HG/NHGRI NIH HHS/United States
GR  - R01 CA119176-05/CA/NCI NIH HHS/United States
GR  - R01 CA118750/CA/NCI NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R01-CA118487/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100708
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Carrier Proteins)
RN  - 0 (Chromatin)
RN  - 0 (Co-Repressor Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histones)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Polycomb-Group Proteins)
RN  - 0 (RCOR1 protein, human)
RN  - 0 (RE1-silencing transcription factor)
RN  - 0 (RNA, Untranslated)
RN  - 0 (Repressor Proteins)
RN  - 0 (SUZ12 protein, human)
RN  - 0 (Transcription Factors)
RN  - EC 1.14.11.- (Histone Demethylases)
RN  - EC 1.5.- (KDM1A protein, human)
RN  - EC 2.1.1.43 (EZH2 protein, human)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
RN  - EC 2.1.1.43 (Polycomb Repressive Complex 2)
SB  - IM
MH  - Binding Sites
MH  - Carrier Proteins/metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chromatin/*metabolism
MH  - Chromatin Immunoprecipitation
MH  - Co-Repressor Proteins
MH  - DNA-Binding Proteins/*metabolism
MH  - Enhancer of Zeste Homolog 2 Protein
MH  - HeLa Cells
MH  - Histone Demethylases/*metabolism
MH  - Histones/*metabolism
MH  - Humans
MH  - Methylation
MH  - Mutation
MH  - Neoplasm Proteins
MH  - Nerve Tissue Proteins/metabolism
MH  - Nuclear Proteins/metabolism
MH  - Nucleic Acid Conformation
MH  - Polycomb Repressive Complex 2
MH  - Polycomb-Group Proteins
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - RNA Interference
MH  - RNA, Untranslated/chemistry/*metabolism
MH  - Repressor Proteins/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
PMC - PMC2967777
MID - NIHMS244741
EDAT- 2010/07/10 06:00
MHDA- 2010/08/21 06:00
PMCR- 2010/11/02
CRDT- 2010/07/10 06:00
PHST- 2010/07/10 06:00 [entrez]
PHST- 2010/07/10 06:00 [pubmed]
PHST- 2010/08/21 06:00 [medline]
PHST- 2010/11/02 00:00 [pmc-release]
AID - science.1192002 [pii]
AID - 10.1126/science.1192002 [doi]
PST - ppublish
SO  - Science. 2010 Aug 6;329(5992):689-93. doi: 10.1126/science.1192002. Epub 2010 Jul 
      8.