PMID- 20647372
OWN - NLM
STAT- MEDLINE
DCOM- 20101202
LR  - 20230523
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 123
IP  - Pt 16
DP  - 2010 Aug 15
TI  - Tao-1 is a negative regulator of microtubule plus-end growth.
PG  - 2708-16
LID - 10.1242/jcs.068726 [doi]
AB  - Microtubule dynamics are dominated by events at microtubule plus ends as they 
      switch between discrete phases of growth and shrinkage. Through their ability to 
      generate force and direct polar cell transport, microtubules help to organise 
      global cell shape and polarity. Conversely, because plus-end binding proteins 
      render the dynamic instability of individual microtubules sensitive to the local 
      intracellular environment, cyto-architecture also affects the overall 
      distribution of microtubules. Despite the importance of plus-end regulation for 
      understanding microtubule cytoskeletal organisation and dynamics, little is known 
      about the signalling mechanisms that trigger changes in their behaviour in space 
      and time. Here, we identify a microtubule-associated kinase, Drosophila Tao-1, as 
      an important regulator of microtubule stability, plus-end dynamics and cell 
      shape. Active Tao-1 kinase leads to the destabilisation of microtubules. 
      Conversely, when Tao-1 function is compromised, rates of cortical-induced 
      microtubule catastrophe are reduced and microtubules contacting the actin cortex 
      continue to elongate, leading to the formation of long microtubule-based 
      protrusions. These data reveal a role for Tao-1 in controlling the dynamic 
      interplay between microtubule plus ends and the actin cortex in the regulation of 
      cell form.
FAU - Liu, Tao
AU  - Liu T
AD  - MRC Laboratory for Molecular Cell Biology, University College London, London, 
      WC1E 6BT, UK.
FAU - Rohn, Jennifer L
AU  - Rohn JL
FAU - Picone, Remigio
AU  - Picone R
FAU - Kunda, Patricia
AU  - Kunda P
FAU - Baum, Buzz
AU  - Baum B
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
GR  - CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100720
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TAO1 protein kinase)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cell Shape/physiology
MH  - Drosophila melanogaster/genetics/growth & development/metabolism
MH  - MAP Kinase Kinase Kinases/genetics/metabolism/*physiology
MH  - Microtubule-Associated Proteins/metabolism/*physiology
MH  - Microtubules/metabolism/*physiology
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - Protein Serine-Threonine Kinases
MH  - RNA, Small Interfering/genetics
PMC - PMC2915876
EDAT- 2010/07/22 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/02/15
CRDT- 2010/07/22 06:00
PHST- 2010/07/22 06:00 [entrez]
PHST- 2010/07/22 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/02/15 00:00 [pmc-release]
AID - jcs.068726 [pii]
AID - 10.1242/jcs.068726 [doi]
PST - ppublish
SO  - J Cell Sci. 2010 Aug 15;123(Pt 16):2708-16. doi: 10.1242/jcs.068726. Epub 2010 
      Jul 20.