PMID- 20842732 OWN - NLM STAT- MEDLINE DCOM- 20110202 LR - 20211203 IS - 1098-2264 (Electronic) IS - 1045-2257 (Linking) VI - 49 IP - 12 DP - 2010 Dec TI - YAP1 and VGLL3, encoding two cofactors of TEAD transcription factors, are amplified and overexpressed in a subset of soft tissue sarcomas. PG - 1161-71 LID - 10.1002/gcc.20825 [doi] AB - In a series of 404 adult soft tissue sarcomas, analyzed by array-CGH, we have observed in approximately 10% of them a genomic amplification of either chromosome bands 11q22 or 3p12. These two amplicons likely target the YAP1 and VGLL3 genes, respectively. Both genes encode proteins that are cofactors of the TEAD family of transcription factors. Very good correlations between amplification and expression levels were observed. Welch test analyses of transcriptome data demonstrate that tumors with amplicons share a large set of upregulated and downregulated genes. Inhibition of YAP1 and VGLL3 in cell lines with these amplifications/overexpressions leads to similar phenotypes: decrease of proliferation rate, and to a lesser extent decrease of migration properties. These data, and the fact that these amplicons are observed either in de-differentiated liposarcomas or in undifferentiated pleomorphic sarcomas, suggest that these genetics events could be involved in oncogenesis and progression of soft tissue sarcomas. CI - (c) 2010 Wiley-Liss, Inc. FAU - Helias-Rodzewicz, Zofia AU - Helias-Rodzewicz Z AD - Genetics and Biology of Cancers, Institut Curie, 26 rue d'Ulm, Paris, France. FAU - Perot, Gaelle AU - Perot G FAU - Chibon, Frederic AU - Chibon F FAU - Ferreira, Celine AU - Ferreira C FAU - Lagarde, Pauline AU - Lagarde P FAU - Terrier, Philippe AU - Terrier P FAU - Coindre, Jean-Michel AU - Coindre JM FAU - Aurias, Alain AU - Aurias A LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Genes Chromosomes Cancer JT - Genes, chromosomes & cancer JID - 9007329 RN - 0 (Adaptor Proteins, Signal Transducing) RN - 0 (Inhibitor of Apoptosis Proteins) RN - 0 (Phosphoproteins) RN - 0 (Transcription Factors) RN - 0 (VGLL3 protein, human) RN - 0 (YAP-Signaling Proteins) RN - 0 (YAP1 protein, human) RN - EC 2.3.2.27 (BIRC2 protein, human) RN - EC 2.3.2.27 (BIRC3 protein, human) RN - EC 2.3.2.27 (Baculoviral IAP Repeat-Containing 3 Protein) RN - EC 2.3.2.27 (Ubiquitin-Protein Ligases) SB - IM MH - Adaptor Proteins, Signal Transducing/*genetics MH - Adult MH - Aged MH - Aged, 80 and over MH - Baculoviral IAP Repeat-Containing 3 Protein MH - Cell Line, Tumor MH - Chromosomes, Human, Pair 11 MH - Chromosomes, Human, Pair 3 MH - Comparative Genomic Hybridization MH - Female MH - *Gene Amplification MH - Gene Expression MH - Gene Expression Profiling MH - Gene Expression Regulation, Neoplastic MH - Humans MH - In Situ Hybridization, Fluorescence MH - Inhibitor of Apoptosis Proteins/genetics MH - Liposarcoma/diagnosis/genetics/pathology MH - Male MH - Middle Aged MH - Phosphoproteins/*genetics MH - Polymerase Chain Reaction MH - Sarcoma/diagnosis/*genetics/pathology MH - Transcription Factors/*genetics MH - Ubiquitin-Protein Ligases MH - YAP-Signaling Proteins EDAT- 2010/09/16 06:00 MHDA- 2011/02/03 06:00 CRDT- 2010/09/16 06:00 PHST- 2010/09/16 06:00 [entrez] PHST- 2010/09/16 06:00 [pubmed] PHST- 2011/02/03 06:00 [medline] AID - 10.1002/gcc.20825 [doi] PST - ppublish SO - Genes Chromosomes Cancer. 2010 Dec;49(12):1161-71. doi: 10.1002/gcc.20825.