PMID- 20858893
OWN - NLM
STAT- MEDLINE
DCOM- 20101230
LR  - 20211203
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 285
IP  - 48
DP  - 2010 Nov 26
TI  - The hippo tumor pathway promotes TAZ degradation by phosphorylating a 
      phosphodegron and recruiting the SCFbeta-TrCP E3 ligase.
PG  - 37159-69
LID - 10.1074/jbc.M110.152942 [doi]
AB  - The TAZ transcription co-activator promotes cell proliferation and 
      epithelial-mesenchymal transition. TAZ is inhibited by the Hippo tumor suppressor 
      pathway, which promotes TAZ cytoplasmic localization by phosphorylation. We 
      report here that TAZ protein stability is controlled by a phosphodegron 
      recognized by the F-box protein beta-TrCP and ubiquitylated by the SCF/CRL1(beta-TrCP) 
      E3 ligase. The interaction between TAZ and beta-TrCP is regulated by the Hippo 
      pathway. Phosphorylation of a phosphodegron in TAZ by LATS primes it for further 
      phosphorylation by CK1epsilon and subsequent binding by beta-TrCP. Therefore, the Hippo 
      pathway negatively regulates TAZ function by both limiting its nuclear 
      accumulation and promoting its degradation. The phosphodegron-mediated TAZ 
      degradation plays an important role in negatively regulating TAZ biological 
      functions.
FAU - Liu, Chen-Ying
AU  - Liu CY
AD  - Key Laboratory of Molecular Medicine, Ministry of Education, Department of 
      Biochemistry and Molecular Biology School of Medicine.
FAU - Zha, Zheng-Yu
AU  - Zha ZY
FAU - Zhou, Xin
AU  - Zhou X
FAU - Zhang, Heng
AU  - Zhang H
FAU - Huang, Wei
AU  - Huang W
FAU - Zhao, Di
AU  - Zhao D
FAU - Li, Tingting
AU  - Li T
FAU - Chan, Siew Wee
AU  - Chan SW
FAU - Lim, Chun Jye
AU  - Lim CJ
FAU - Hong, Wanjin
AU  - Hong W
FAU - Zhao, Shimin
AU  - Zhao S
FAU - Xiong, Yue
AU  - Xiong Y
FAU - Lei, Qun-Ying
AU  - Lei QY
FAU - Guan, Kun-Liang
AU  - Guan KL
LA  - eng
GR  - R01 CA132809/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100921
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Drosophila Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Transcriptional Coactivator with PDZ-Binding Motif Proteins)
RN  - 0 (WWTR1 protein, human)
RN  - EC 2.3.2.27 (SKP Cullin F-Box Protein Ligases)
RN  - EC 2.7.1.- (LATS1 protein, human)
RN  - EC 2.7.11.1 (Casein Kinase I)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (hpo protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Casein Kinase I/genetics/metabolism
MH  - Drosophila Proteins/genetics/metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/chemistry/genetics/*metabolism
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Protein Stability
MH  - SKP Cullin F-Box Protein Ligases/genetics/*metabolism
MH  - *Signal Transduction
MH  - Trans-Activators
MH  - Transcription Factors
MH  - Transcriptional Coactivator with PDZ-Binding Motif Proteins
PMC - PMC2988322
EDAT- 2010/09/23 06:00
MHDA- 2010/12/31 06:00
PMCR- 2011/11/26
CRDT- 2010/09/23 06:00
PHST- 2010/09/23 06:00 [entrez]
PHST- 2010/09/23 06:00 [pubmed]
PHST- 2010/12/31 06:00 [medline]
PHST- 2011/11/26 00:00 [pmc-release]
AID - S0021-9258(20)46692-X [pii]
AID - M110.152942 [pii]
AID - 10.1074/jbc.M110.152942 [doi]
PST - ppublish
SO  - J Biol Chem. 2010 Nov 26;285(48):37159-69. doi: 10.1074/jbc.M110.152942. Epub 
      2010 Sep 21.