PMID- 20885979
OWN - NLM
STAT- MEDLINE
DCOM- 20110218
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 5
IP  - 9
DP  - 2010 Sep 24
TI  - Curcumin protects intestinal mucosal barrier function of rat enteritis via 
      activation of MKP-1 and attenuation of p38 and NF-kappaB activation.
PG  - e12969
LID - e12969 [pii]
LID - 10.1371/journal.pone.0012969 [doi]
AB  - BACKGROUND: Intestinal mucosa barrier (IMB) dysfunction results in many notorious 
      diseases for which there are currently few effective treatments. We studied 
      curcumin's protective effect on IMB and examined its mechanism by using 
      methotrexate (MTX) induced rat enteritis model and lipopolysaccharide (LPS) 
      treated cell death model. METHODOLOGY/PRINCIPAL FINDINGS: Curcumin was 
      intragastrically administrated from the first day, models were made for 7 days. 
      Cells were treated with curcumin for 30 min before exposure to LPS. Rat 
      intestinal mucosa was collected for evaluation of pathological changes. We 
      detected the activities of D-lactate and diamine oxidase (DAO) according to 
      previous research and measured the levels of myeloperoxidase (MPO) and superoxide 
      dismutase (SOD) by colorimetric method. Intercellular adhesion molecule-1 
      (ICAM-1), tumor necrosis factor alpha (TNF-alpha) and interleukin 1beta (IL-1beta) were 
      determined by RT-PCR and IL-10 production was determined by ELISA. We found 
      Curcumin decreased the levels of D-lactate, DAO, MPO, ICAM-1, IL-1beta and TNF-alpha, 
      but increased the levels of IL-10 and SOD in rat models. We further confirmed 
      mitogen-activated protein kinase phosphatase-1 (MKP-1) was activated but 
      phospho-p38 was inhibited by curcumin by western blot assay. Finally, NF-kappaB 
      translocation was monitored by immunofluorescent staining. We showed that 
      curcumin repressed I-kappaB and interfered with the translocation of NF-kappaB into 
      nucleus. CONCLUSIONS/SIGNIFICANCE: The effect of curcumin is mediated by the 
      MKP-1-dependent inactivation of p38 and inhibition of NF-kappaB-mediated 
      transcription. Curcumin, with anti-inflammatory and anti-oxidant activities may 
      be used as an effective reagent for protecting intestinal mucosa barrier and 
      other related intestinal diseases.
FAU - Song, Wei-Bing
AU  - Song WB
AD  - Department of Gerontology, General Hospital of Guangzhou Military Command of 
      People's Liberation Army, Guangzhou, China. songweibing@yahoo.com
FAU - Wang, Yuan-Yuan
AU  - Wang YY
FAU - Meng, Fan-Su
AU  - Meng FS
FAU - Zhang, Qing-Hua
AU  - Zhang QH
FAU - Zeng, Jian-Ying
AU  - Zeng JY
FAU - Xiao, Li-Ping
AU  - Xiao LP
FAU - Yu, Xin-Pei
AU  - Yu XP
FAU - Peng, Dan-dan
AU  - Peng DD
FAU - Su, Lei
AU  - Su L
FAU - Xiao, Bing
AU  - Xiao B
FAU - Zhang, Zhen-Shu
AU  - Zhang ZS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100924
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (NF-kappa B)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.1.3.48 (Dual Specificity Phosphatase 1)
RN  - EC 3.1.3.48 (Dusp1 protein, rat)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Curcumin/*administration & dosage
MH  - Disease Models, Animal
MH  - Dual Specificity Phosphatase 1/genetics/*metabolism
MH  - Enteritis/*drug therapy/enzymology/genetics/metabolism
MH  - Enzyme Activation/drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Intestinal Mucosa/*drug effects/enzymology/metabolism
MH  - Mice
MH  - NF-kappa B/genetics/*metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - p38 Mitogen-Activated Protein Kinases/genetics/*metabolism
PMC - PMC2945766
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2010/10/05 06:00
MHDA- 2011/02/22 06:00
PMCR- 2010/09/24
CRDT- 2010/10/02 06:00
PHST- 2010/07/08 00:00 [received]
PHST- 2010/09/01 00:00 [accepted]
PHST- 2010/10/02 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2011/02/22 06:00 [medline]
PHST- 2010/09/24 00:00 [pmc-release]
AID - e12969 [pii]
AID - 10-PONE-RA-20838R1 [pii]
AID - 10.1371/journal.pone.0012969 [doi]
PST - epublish
SO  - PLoS One. 2010 Sep 24;5(9):e12969. doi: 10.1371/journal.pone.0012969.