PMID- 20887898
OWN - NLM
STAT- MEDLINE
DCOM- 20101019
LR  - 20220331
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Linking)
VI  - 143
IP  - 1
DP  - 2010 Oct 1
TI  - Intestinal crypt homeostasis results from neutral competition between 
      symmetrically dividing Lgr5 stem cells.
PG  - 134-44
LID - 10.1016/j.cell.2010.09.016 [doi]
AB  - Intestinal stem cells, characterized by high Lgr5 expression, reside between 
      Paneth cells at the small intestinal crypt base and divide every day. We have 
      carried out fate mapping of individual stem cells by generating a multicolor 
      Cre-reporter. As a population, Lgr5(hi) stem cells persist life-long, yet crypts 
      drift toward clonality within a period of 1-6 months. We have collected short- 
      and long-term clonal tracing data of individual Lgr5(hi) cells. These reveal that 
      most Lgr5(hi) cell divisions occur symmetrically and do not support a model in 
      which two daughter cells resulting from an Lgr5(hi) cell division adopt divergent 
      fates (i.e., one Lgr5(hi) cell and one transit-amplifying [TA] cell per 
      division). The cellular dynamics are consistent with a model in which the 
      resident stem cells double their numbers each day and stochastically adopt stem 
      or TA fates. Quantitative analysis shows that stem cell turnover follows a 
      pattern of neutral drift dynamics.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Snippert, Hugo J
AU  - Snippert HJ
AD  - Hubrecht Institute, KNAW and University Medical Center Utrecht, Uppsalalaan 8, 
      3584 CT Utrecht, The Netherlands.
FAU - van der Flier, Laurens G
AU  - van der Flier LG
FAU - Sato, Toshiro
AU  - Sato T
FAU - van Es, Johan H
AU  - van Es JH
FAU - van den Born, Maaike
AU  - van den Born M
FAU - Kroon-Veenboer, Carla
AU  - Kroon-Veenboer C
FAU - Barker, Nick
AU  - Barker N
FAU - Klein, Allon M
AU  - Klein AM
FAU - van Rheenen, Jacco
AU  - van Rheenen J
FAU - Simons, Benjamin D
AU  - Simons BD
FAU - Clevers, Hans
AU  - Clevers H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Lgr5 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
CIN - Dev Cell. 2010 Oct 19;19(4):489-90. doi: 10.1016/j.devcel.2010.10.001. PMID: 
      20951341
CIN - Nat Rev Mol Cell Biol. 2010 Nov;11(11):752. doi: 10.1038/nrm3004. PMID: 20966965
MH  - Animals
MH  - *Cell Lineage
MH  - Clone Cells
MH  - Intestine, Small/*cytology
MH  - Mice
MH  - Models, Biological
MH  - Receptors, G-Protein-Coupled/metabolism
MH  - Stem Cells/*cytology
EDAT- 2010/10/05 06:00
MHDA- 2010/10/20 06:00
CRDT- 2010/10/05 06:00
PHST- 2010/07/15 00:00 [received]
PHST- 2010/09/07 00:00 [revised]
PHST- 2010/09/10 00:00 [accepted]
PHST- 2010/10/05 06:00 [entrez]
PHST- 2010/10/05 06:00 [pubmed]
PHST- 2010/10/20 06:00 [medline]
AID - S0092-8674(10)01064-0 [pii]
AID - 10.1016/j.cell.2010.09.016 [doi]
PST - ppublish
SO  - Cell. 2010 Oct 1;143(1):134-44. doi: 10.1016/j.cell.2010.09.016.