PMID- 20937699
OWN - NLM
STAT- MEDLINE
DCOM- 20110222
LR  - 20230406
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 191
IP  - 2
DP  - 2010 Oct 18
TI  - RAD18-mediated ubiquitination of PCNA activates the Fanconi anemia DNA repair 
      network.
PG  - 249-57
LID - 10.1083/jcb.201005101 [doi]
AB  - The Fanconi anemia (FA) network is important for the repair of interstrand DNA 
      cross-links. A key event in FA pathway activation is the monoubiquitylation of 
      the FA complementation group I (FANCI)-FANCD2 (ID) complex by FA complementation 
      group L (FANCL), an E3 ubiquitin ligase. In this study, we show that RAD18, 
      another DNA damage-activated E3 ubiquitin ligase, also participates in ID complex 
      activation by ubiquitylating proliferating cell nuclear antigen (PCNA) on Lys164, 
      an event required for the recruitment of FANCL to chromatin. We also found that 
      monoubiquitylated PCNA stimulates FANCL-catalyzed FANCD2 and FANCI 
      monoubiquitylation. Collectively, these experiments identify RAD18-mediated PCNA 
      monoubiquitination as a central hub for the mobilization of the FA pathway by 
      promoting FANCL-mediated FANCD2 monoubiquitylation.
FAU - Geng, Liyi
AU  - Geng L
AD  - Division of Oncology Research, Mayo Clinic College of Medicine, Rochester, MN 
      55905, USA.
FAU - Huntoon, Catherine J
AU  - Huntoon CJ
FAU - Karnitz, Larry M
AU  - Karnitz LM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101011
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Chromatin)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FANCD2 protein, human)
RN  - 0 (Fanconi Anemia Complementation Group D2 Protein)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RAD18 protein, human)
RN  - EC 2.3.2.27 (FANCL protein, human)
RN  - EC 2.3.2.27 (Fanconi Anemia Complementation Group L Protein)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Binding Sites
MH  - Chromatin/metabolism
MH  - Cisplatin/pharmacology
MH  - Cross-Linking Reagents/pharmacology
MH  - *DNA Repair
MH  - DNA-Binding Proteins/genetics/metabolism/*physiology
MH  - Fanconi Anemia Complementation Group D2 Protein/genetics/metabolism/*physiology
MH  - Fanconi Anemia Complementation Group L Protein/chemistry/metabolism/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Models, Genetic
MH  - Proliferating Cell Nuclear Antigen/chemistry/*metabolism
MH  - Protein Structure, Tertiary
MH  - RNA Interference
MH  - Ubiquitin-Protein Ligases
MH  - Ubiquitination
PMC - PMC2958487
EDAT- 2010/10/13 06:00
MHDA- 2011/02/23 06:00
PMCR- 2011/04/18
CRDT- 2010/10/13 06:00
PHST- 2010/10/13 06:00 [entrez]
PHST- 2010/10/13 06:00 [pubmed]
PHST- 2011/02/23 06:00 [medline]
PHST- 2011/04/18 00:00 [pmc-release]
AID - jcb.201005101 [pii]
AID - 201005101 [pii]
AID - 10.1083/jcb.201005101 [doi]
PST - ppublish
SO  - J Cell Biol. 2010 Oct 18;191(2):249-57. doi: 10.1083/jcb.201005101. Epub 2010 Oct 
      11.