PMID- 21040900
OWN - NLM
STAT- MEDLINE
DCOM- 20110310
LR  - 20240109
IS  - 1875-9777 (Electronic)
IS  - 1934-5909 (Print)
IS  - 1875-9777 (Linking)
VI  - 7
IP  - 5
DP  - 2010 Nov 5
TI  - The steroid hormone ecdysone functions with intrinsic chromatin remodeling 
      factors to control female germline stem cells in Drosophila.
PG  - 581-92
LID - 10.1016/j.stem.2010.10.001 [doi]
AB  - Steroid hormones are known systemic regulators of multiple normal and cancerous 
      tissues; however, whether or how they impact the fate and function of adult stem 
      cells is unclear. In the Drosophila ovary, insulin signals modulate the 
      proliferation and self-renewal of germline stem cells (GSCs), yet despite 
      evidence that additional systemic factors control GSC activity, these have 
      remained largely unknown. Here, we report that ecdysone, a steroid hormone 
      structurally related to mammalian sex steroids, directly regulates adult GSC 
      proliferation and self-renewal independently of insulin signaling. Ecdysone 
      controls GSCs through a functional interaction with the chromatin remodeling 
      factors ISWI, an intrinsic epigenetic factor required for GSC fate and activity, 
      and Nurf301, the largest subunit of the ISWI-containing NURF chromatin remodeling 
      complex. Our findings support a link between systemic steroid hormones and the 
      intrinsic chromatin remodeling machinery as a potential mechanism to promote 
      broad transcriptional programs required for adult stem cell self-renewal.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Ables, Elizabeth T
AU  - Ables ET
AD  - Department of Cell and Developmental Biology, Vanderbilt University Medical 
      Center, Nashville, TN, 37232, USA.
FAU - Drummond-Barbosa, Daniela
AU  - Drummond-Barbosa D
LA  - eng
GR  - R01 GM069875-05/GM/NIGMS NIH HHS/United States
GR  - R01 GM069875/GM/NIGMS NIH HHS/United States
GR  - F32 GM086031/GM/NIGMS NIH HHS/United States
GR  - R01 GM069875-03/GM/NIGMS NIH HHS/United States
GR  - T32 DK007563/DK/NIDDK NIH HHS/United States
GR  - R01 GM069875-04/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Stem Cell
JT  - Cell stem cell
JID - 101311472
RN  - 3604-87-3 (Ecdysone)
SB  - IM
MH  - Animals
MH  - Cell Cycle/physiology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chromatin Assembly and Disassembly/*genetics
MH  - *Drosophila
MH  - Ecdysone/genetics/*metabolism/*pharmacology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Germ Cells/cytology/*metabolism
MH  - Signal Transduction
MH  - Stem Cells/cytology/*metabolism
PMC - PMC3292427
MID - NIHMS245613
COIS- The authors declare no competing conflicts of interest.
EDAT- 2010/11/03 06:00
MHDA- 2011/03/11 06:00
PMCR- 2012/03/02
CRDT- 2010/11/03 06:00
PHST- 2010/02/19 00:00 [received]
PHST- 2010/08/12 00:00 [revised]
PHST- 2010/10/06 00:00 [accepted]
PHST- 2010/11/03 06:00 [entrez]
PHST- 2010/11/03 06:00 [pubmed]
PHST- 2011/03/11 06:00 [medline]
PHST- 2012/03/02 00:00 [pmc-release]
AID - S1934-5909(10)00514-X [pii]
AID - 10.1016/j.stem.2010.10.001 [doi]
PST - ppublish
SO  - Cell Stem Cell. 2010 Nov 5;7(5):581-92. doi: 10.1016/j.stem.2010.10.001.