PMID- 21074439
OWN - NLM
STAT- MEDLINE
DCOM- 20110314
LR  - 20211020
IS  - 1879-0445 (Electronic)
IS  - 0960-9822 (Print)
IS  - 0960-9822 (Linking)
VI  - 20
IP  - 23
DP  - 2010 Dec 7
TI  - Developmental control of late replication and S phase length.
PG  - 2067-77
LID - 10.1016/j.cub.2010.10.021 [doi]
AB  - BACKGROUND: Fast, early embryonic cell cycles have correspondingly fast S phases. 
      In early Drosophila embryos, forks starting from closely spaced origins replicate 
      the whole genome in 3.4 min, ten times faster than in embryonic cycle 14 and a 
      hundred times faster than in a wing disc. It is not known how S phase duration is 
      regulated. Here we examined prolongation of embryonic S phases, its coupling to 
      development, and its relationship to the appearance of heterochromatin. RESULTS: 
      Imaging of fluorescent nucleotide incorporation and GFP-PCNA gave exquisite time 
      resolution of S phase events. In the early S phases, satellite sequences 
      replicated rapidly despite a compact chromatin structure. In S phases 11-13, a 
      delay in satellite replication emerged in sync with modest and progressive 
      prolongation of S phase. In S phase 14, major and distinct delays ordered the 
      replication of satellites into a sequence that occupied much of S phase. This 
      onset of late replication required transcription. Satellites only accumulated 
      abundant heterochromatin protein 1 (HP1) after replicating in S phase 14. By 
      cycle 15, satellites clustered in a compact HP1-positive mass, but replication 
      occurred at decondensed foci at the surface of this mass. CONCLUSIONS: The 
      slowing of S phase is an active process, not a titration of maternal replication 
      machinery. Most sequences continue to replicate rapidly in successive cycles, but 
      increasing delays in the replication of satellite sequences extend S phase. 
      Although called constitutively heterochromatic, satellites acquire the 
      distinctive features of heterochromatin, compaction, late replication, HP1 
      binding, and aggregation at the chromocenter, in successive steps coordinated 
      with developmental progress.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Shermoen, Antony W
AU  - Shermoen AW
AD  - Department of Biochemistry & Biophysics, University of California, San Francisco, 
      San Francisco, CA 94143-2200, USA.
FAU - McCleland, Mark L
AU  - McCleland ML
FAU - O'Farrell, Patrick H
AU  - O'Farrell PH
LA  - eng
GR  - R01 GM037193-20/GM/NIGMS NIH HHS/United States
GR  - GM037193/GM/NIGMS NIH HHS/United States
GR  - R01 GM037193-24/GM/NIGMS NIH HHS/United States
GR  - R01 GM037193-22/GM/NIGMS NIH HHS/United States
GR  - R01 GM037193-21/GM/NIGMS NIH HHS/United States
GR  - GM078710/GM/NIGMS NIH HHS/United States
GR  - R01 GM037193-23/GM/NIGMS NIH HHS/United States
GR  - R01 GM037193/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20101111
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA, Satellite)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Heterochromatin)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (heterochromatin protein 1, Drosophila)
SB  - IM
CIN - Curr Biol. 2010 Dec 7;20(23):R1018-20. PMID: 21145016
MH  - Animals
MH  - Chromosomal Proteins, Non-Histone/genetics/metabolism
MH  - *DNA Replication
MH  - DNA, Satellite/genetics/metabolism
MH  - Drosophila Proteins/genetics/metabolism
MH  - Drosophila melanogaster/*embryology/*genetics
MH  - Heterochromatin/metabolism
MH  - Proliferating Cell Nuclear Antigen/genetics/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - *S Phase
PMC - PMC3108027
MID - NIHMS253142
EDAT- 2010/11/16 06:00
MHDA- 2011/03/15 06:00
PMCR- 2011/12/07
CRDT- 2010/11/16 06:00
PHST- 2010/08/19 00:00 [received]
PHST- 2010/10/05 00:00 [revised]
PHST- 2010/10/07 00:00 [accepted]
PHST- 2010/11/16 06:00 [entrez]
PHST- 2010/11/16 06:00 [pubmed]
PHST- 2011/03/15 06:00 [medline]
PHST- 2011/12/07 00:00 [pmc-release]
AID - S0960-9822(10)01285-6 [pii]
AID - 10.1016/j.cub.2010.10.021 [doi]
PST - ppublish
SO  - Curr Biol. 2010 Dec 7;20(23):2067-77. doi: 10.1016/j.cub.2010.10.021. Epub 2010 
      Nov 11.