PMID- 2110186
OWN - NLM
STAT- MEDLINE
DCOM- 19900606
LR  - 20240104
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 27
IP  - 2-3
DP  - 1990 May
TI  - Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus.
PG  - 229-37
AB  - A murine cell line (BV-2) has been generated by infecting primary microglial cell 
      cultures with a v-raf/v-myc oncogene carrying retrovirus (J2). BV-2 cells 
      expressed nonspecific esterase activity, phagocytic ability and lacked peroxidase 
      activity. Such cells secreted lysozyme and, following appropriate stimulation, 
      also interleukin 1 and tumor necrosis factor. Furthermore, BV-2 cells exhibited 
      spontaneous anti-Candida activity and acquired tumoricidal activity upon 
      treatment with interferon-gamma. Phenotypically, BV-2 cells resulted positive for 
      MAC1 and MAC2 antigens, and negative for MAC3, glial fibrillary acidic protein 
      (GFAP) and galactocerebroside (GC) antigens. Since BV-2 cells retain most of the 
      morphological, phenotypical and functional properties described for freshly 
      isolated microglial cells, we can conclude that J2 virus infection has resulted 
      in the immortalization of active microglial cells.
FAU - Blasi, E
AU  - Blasi E
AD  - Department of Experimental Medicine and Biochemical Sciences, University of 
      Perugia, Italy.
FAU - Barluzzi, R
AU  - Barluzzi R
FAU - Bocchini, V
AU  - Bocchini V
FAU - Mazzolla, R
AU  - Mazzolla R
FAU - Bistoni, F
AU  - Bistoni F
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - *Cell Transformation, Viral
MH  - Cells, Cultured
MH  - Interferon-gamma/pharmacology
MH  - Macrophages/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuroglia/*physiology
MH  - *Oncogenes
MH  - Phagocytosis
MH  - Phenotype
MH  - Retroviridae/*genetics
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1990/05/01 00:00
MHDA- 2000/06/01 09:00
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 2000/06/01 09:00 [medline]
PHST- 1990/05/01 00:00 [entrez]
AID - 0165-5728(90)90073-V [pii]
AID - 10.1016/0165-5728(90)90073-v [doi]
PST - ppublish
SO  - J Neuroimmunol. 1990 May;27(2-3):229-37. doi: 10.1016/0165-5728(90)90073-v.