PMID- 21106759
OWN - NLM
STAT- MEDLINE
DCOM- 20110512
LR  - 20240104
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 107
IP  - 50
DP  - 2010 Dec 14
TI  - Histone H3K27ac separates active from poised enhancers and predicts developmental 
      state.
PG  - 21931-6
LID - 10.1073/pnas.1016071107 [doi]
AB  - Developmental programs are controlled by transcription factors and chromatin 
      regulators, which maintain specific gene expression programs through epigenetic 
      modification of the genome. These regulatory events at enhancers contribute to 
      the specific gene expression programs that determine cell state and the potential 
      for differentiation into new cell types. Although enhancer elements are known to 
      be associated with certain histone modifications and transcription factors, the 
      relationship of these modifications to gene expression and developmental state 
      has not been clearly defined. Here we interrogate the epigenetic landscape of 
      enhancer elements in embryonic stem cells and several adult tissues in the mouse. 
      We find that histone H3K27ac distinguishes active enhancers from inactive/poised 
      enhancer elements containing H3K4me1 alone. This indicates that the amount of 
      actively used enhancers is lower than previously anticipated. Furthermore, poised 
      enhancer networks provide clues to unrealized developmental programs. Finally, we 
      show that enhancers are reset during nuclear reprogramming.
FAU - Creyghton, Menno P
AU  - Creyghton MP
AD  - Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
FAU - Cheng, Albert W
AU  - Cheng AW
FAU - Welstead, G Grant
AU  - Welstead GG
FAU - Kooistra, Tristan
AU  - Kooistra T
FAU - Carey, Bryce W
AU  - Carey BW
FAU - Steine, Eveline J
AU  - Steine EJ
FAU - Hanna, Jacob
AU  - Hanna J
FAU - Lodato, Michael A
AU  - Lodato MA
FAU - Frampton, Garrett M
AU  - Frampton GM
FAU - Sharp, Phillip A
AU  - Sharp PA
FAU - Boyer, Laurie A
AU  - Boyer LA
FAU - Young, Richard A
AU  - Young RA
FAU - Jaenisch, Rudolf
AU  - Jaenisch R
LA  - eng
SI  - GEO/GSE23907
SI  - GEO/GSE24164
SI  - GEO/GSE24165
GR  - 5-RO1-CA87869/CA/NCI NIH HHS/United States
GR  - P01 CA042063/CA/NCI NIH HHS/United States
GR  - P30-CA14051/CA/NCI NIH HHS/United States
GR  - 5-R37-CA084198/CA/NCI NIH HHS/United States
GR  - R37 HD045022/HD/NICHD NIH HHS/United States
GR  - R37 CA084198/CA/NCI NIH HHS/United States
GR  - P01-CA42063/CA/NCI NIH HHS/United States
GR  - R01 CA084198/CA/NCI NIH HHS/United States
GR  - HG002668/HG/NHGRI NIH HHS/United States
GR  - P30 CA014051/CA/NCI NIH HHS/United States
GR  - 5-RO1-HDO45022/PHS HHS/United States
GR  - R01 HG002668/HG/NHGRI NIH HHS/United States
GR  - R01 CA087869/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101124
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Histones)
SB  - IM
CIN - Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21240-1. PMID: 21135244
MH  - Acetylation
MH  - Animals
MH  - Cell Differentiation/genetics
MH  - Cell Line
MH  - *Enhancer Elements, Genetic
MH  - *Gene Expression Regulation, Developmental
MH  - Histones/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
PMC - PMC3003124
COIS- The authors declare no conflict of interest.
EDAT- 2010/11/26 06:00
MHDA- 2011/05/13 06:00
PMCR- 2011/06/14
CRDT- 2010/11/26 06:00
PHST- 2010/11/26 06:00 [entrez]
PHST- 2010/11/26 06:00 [pubmed]
PHST- 2011/05/13 06:00 [medline]
PHST- 2011/06/14 00:00 [pmc-release]
AID - 1016071107 [pii]
AID - 201016071 [pii]
AID - 10.1073/pnas.1016071107 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21931-6. doi: 
      10.1073/pnas.1016071107. Epub 2010 Nov 24.