PMID- 21145745
OWN - NLM
STAT- MEDLINE
DCOM- 20110404
LR  - 20211020
IS  - 1879-0445 (Electronic)
IS  - 0960-9822 (Linking)
VI  - 20
IP  - 24
DP  - 2010 Dec 21
TI  - Cnn dynamics drive centrosome size asymmetry to ensure daughter centriole 
      retention in Drosophila neuroblasts.
PG  - 2187-92
LID - 10.1016/j.cub.2010.11.055 [doi]
AB  - Centrosomes comprise a pair of centrioles surrounded by an amorphous network of 
      pericentriolar material (PCM). In certain stem cells, the two centrosomes differ 
      in size, and this appears to be important for asymmetric cell division [1, 2]. In 
      some cases, centrosome asymmetry is linked to centriole age because the older, 
      mother centriole always organizes more PCM than the daughter centriole, thus 
      ensuring that the mother centriole is always retained in the stem cell after cell 
      division [3]. This has raised the possibility that an "immortal" mother centriole 
      may help maintain stem cell fate [4, 5]. It is unclear, however, how centrosome 
      size asymmetry is generated in stem cells. Here we provide compelling evidence 
      that centrosome size asymmetry in Drosophila neuroblasts is generated by the 
      differential regulation of Cnn incorporation into the PCM at mother and daughter 
      centrioles. Shortly after centriole separation, mother and daughter centrioles 
      organize similar amounts of PCM, but Cnn incorporation is then rapidly 
      downregulated at the mother centriole, while it is maintained at the daughter 
      centriole. This ensures that the daughter centriole maintains its PCM and so its 
      position at the apical cortex. Thus, the daughter centriole, rather than an 
      "immortal" mother centriole, is ultimately retained in these stem cells.
FAU - Conduit, Paul T
AU  - Conduit PT
AD  - Sir William Dunn School of Pathology, University of Oxford, South Parks Road, 
      Oxford OX1 3RE, UK.
FAU - Raff, Jordan W
AU  - Raff JW
LA  - eng
GR  - 10530/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101209
PL  - England
TA  - Curr Biol
JT  - Current biology : CB
JID - 9107782
RN  - 0 (Drosophila Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (cnn protein, Drosophila)
SB  - IM
MH  - Animals
MH  - Centrioles/metabolism
MH  - Centrosome/metabolism
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Drosophila melanogaster/*cytology/embryology
MH  - Fluorescence Recovery After Photobleaching
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Humans
MH  - Neurons/*cytology/physiology
MH  - Recombinant Fusion Proteins/genetics/metabolism
EDAT- 2010/12/15 06:00
MHDA- 2011/04/05 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/08/23 00:00 [received]
PHST- 2010/10/26 00:00 [revised]
PHST- 2010/11/19 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2011/04/05 06:00 [medline]
AID - S0960-9822(10)01521-6 [pii]
AID - 10.1016/j.cub.2010.11.055 [doi]
PST - ppublish
SO  - Curr Biol. 2010 Dec 21;20(24):2187-92. doi: 10.1016/j.cub.2010.11.055. Epub 2010 
      Dec 9.