PMID- 21157891
OWN - NLM
STAT- MEDLINE
DCOM- 20110121
LR  - 20220316
IS  - 1545-5017 (Electronic)
IS  - 1545-5009 (Linking)
VI  - 56
IP  - 2
DP  - 2011 Feb
TI  - Survey of MicroRNA expression in pediatric brain tumors.
PG  - 211-6
LID - 10.1002/pbc.22723 [doi]
AB  - BACKGROUND: A better understanding of pediatric brain tumor biology is needed to 
      assist in the development of less toxic therapies and to provide better markers 
      for disease stratification. MicroRNAs (miRNA) may play a significant role in 
      brain tumor biology. The present study provides an initial survey of miRNA 
      expression in pediatric central nervous system (CNS) malignancies including 
      atypical teratoid/rhabdoid tumor, ependymoma, glioblastoma, medulloblastoma, and 
      pilocytic astrocytoma. PROCEDURE: MicroRNA expression in pediatric brain tumors 
      and normal tissue controls was examined by microarray. Three aberrantly expressed 
      miRNAs were further studied in a larger cohort by quantitative real-time PCR 
      (qRT-PCR). RESULTS: MicroRNA-129, miR-142-5p, and miR-25 were differentially 
      expressed in every pediatric brain tumor type compared to normal tissue controls 
      as measured by microarray. When further examined by qRT-PCR, these miRNAs 
      demonstrated differential expression that significantly correlated with the 
      microarray findings. Distinctive miRNA expression profiles were also observed in 
      the different pediatric brain tumor types. CONCLUSIONS: MicroRNAs are 
      differentially expressed between pediatric CNS neoplasms and normal tissue 
      suggesting that they may play a significant role in oncogenesis. A greater 
      understanding of aberrant miRNA expression in pediatric brain tumors may aid in 
      the development of novel therapies. The characterization of tumor-specific miRNA 
      signatures may aid in the discovery of biomarkers with diagnostic or prognostic 
      utility.
CI  - Copyright (c) 2010 Wiley-Liss, Inc.
FAU - Birks, Diane K
AU  - Birks DK
AD  - Department of Neurosurgery, Anschutz Medical Campus, University of Colorado at 
      Denver, Aurora, Colorado 80045, USA. diane.birks@ucdenver.edu
FAU - Barton, Valerie N
AU  - Barton VN
FAU - Donson, Andrew M
AU  - Donson AM
FAU - Handler, Michael H
AU  - Handler MH
FAU - Vibhakar, Rajeev
AU  - Vibhakar R
FAU - Foreman, Nicholas K
AU  - Foreman NK
LA  - eng
GR  - K08 NS059790/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101103
PL  - United States
TA  - Pediatr Blood Cancer
JT  - Pediatric blood & cancer
JID - 101186624
RN  - 0 (MicroRNAs)
SB  - IM
CIN - Pediatr Blood Cancer. 2011 Jul 15;57(1):183-4. PMID: 21425446
MH  - Brain Neoplasms/*genetics
MH  - Child
MH  - Child, Preschool
MH  - Cluster Analysis
MH  - Gene Expression
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - MicroRNAs/*biosynthesis
MH  - Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2010/12/16 06:00
MHDA- 2011/01/22 06:00
CRDT- 2010/12/16 06:00
PHST- 2010/04/12 00:00 [received]
PHST- 2010/05/28 00:00 [accepted]
PHST- 2010/12/16 06:00 [entrez]
PHST- 2010/12/16 06:00 [pubmed]
PHST- 2011/01/22 06:00 [medline]
AID - 10.1002/pbc.22723 [doi]
PST - ppublish
SO  - Pediatr Blood Cancer. 2011 Feb;56(2):211-6. doi: 10.1002/pbc.22723. Epub 2010 Nov 
      3.