PMID- 21163928
OWN - NLM
STAT- MEDLINE
DCOM- 20110331
LR  - 20211020
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 117
IP  - 7
DP  - 2011 Feb 17
TI  - Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human 
      embryonic stem cells.
PG  - 2157-65
LID - 10.1182/blood-2010-08-302711 [doi]
AB  - MicroRNAs (miRs) play an important role in cell differentiation and maintenance 
      of cell identity, but relatively little is known of their functional role in 
      modulating human hematopoietic lineage differentiation. Human embryonic stem 
      cells (hESCs) provide a model system to study early human hematopoiesis. We 
      differentiated hESCs by embryoid body (EB) formation and compared the miR 
      expression profile of undifferentiated hESCs to CD34(+) EB cells. miRs-126/126* 
      were the most enriched of the 7 miRs that were up-regulated in CD34(+) cells, and 
      their expression paralleled the kinetics of hematopoietic transcription factors 
      RUNX1, SCL, and PU.1. To define the role of miRs-126/126* in hematopoiesis, we 
      created hESCs overexpressing doxycycline-regulated miRs-126/126* and analyzed 
      their hematopoietic differentiation. Induction of miRs-126/126* during both EB 
      differentiation and colony formation reduced the number of erythroid colonies, 
      suggesting an inhibitory role of miRs-126/126* in erythropoiesis. Protein 
      tyrosine phosphatase, nonreceptor type 9 (PTPN9), a protein tyrosine phosphatase 
      that is required for growth and expansion of erythroid cells, is one target of 
      miR-126. PTPN9 restoration partially relieved the suppressed erythropoiesis 
      caused by miRs-126/126*. Our results define an important function of 
      miRs-126/126* in negative regulation of erythropoiesis, providing the first 
      evidence for a role of miR in hematopoietic differentiation of hESCs.
FAU - Huang, Xinqiang
AU  - Huang X
AD  - Howard Hughes Medical Institute, Los Angeles, CA, USA.
FAU - Gschweng, Eric
AU  - Gschweng E
FAU - Van Handel, Ben
AU  - Van Handel B
FAU - Cheng, Donghui
AU  - Cheng D
FAU - Mikkola, Hanna K A
AU  - Mikkola HK
FAU - Witte, Owen N
AU  - Witte ON
LA  - eng
GR  - T32 GM007185/GM/NIGMS NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - GM007185/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101216
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD34)
RN  - 0 (DNA Primers)
RN  - 0 (MIRN126 microRNA, human)
RN  - 0 (MIRN223 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 3.1.3.48 (PTPN9 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases, Non-Receptor)
SB  - IM
MH  - Antigens, CD34/metabolism
MH  - Base Sequence
MH  - Cell Differentiation/genetics/physiology
MH  - Cell Line
MH  - Colony-Forming Units Assay
MH  - DNA Primers/genetics
MH  - Embryoid Bodies/cytology/metabolism
MH  - Embryonic Stem Cells/*cytology/*metabolism
MH  - Erythropoiesis/*genetics/*physiology
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Kinetics
MH  - MicroRNAs/*genetics/metabolism
MH  - Protein Tyrosine Phosphatases, Non-Receptor/genetics/physiology
PMC - PMC3062325
EDAT- 2010/12/18 06:00
MHDA- 2011/04/01 06:00
PMCR- 2011/02/17
CRDT- 2010/12/18 06:00
PHST- 2010/12/18 06:00 [entrez]
PHST- 2010/12/18 06:00 [pubmed]
PHST- 2011/04/01 06:00 [medline]
PHST- 2011/02/17 00:00 [pmc-release]
AID - S0006-4971(20)55096-9 [pii]
AID - 2010/302711 [pii]
AID - 10.1182/blood-2010-08-302711 [doi]
PST - ppublish
SO  - Blood. 2011 Feb 17;117(7):2157-65. doi: 10.1182/blood-2010-08-302711. Epub 2010 
      Dec 16.