PMID- 21173232
OWN - NLM
STAT- MEDLINE
DCOM- 20110210
LR  - 20220318
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 108
IP  - 1
DP  - 2011 Jan 4
TI  - Mouse telomerase reverse transcriptase (mTert) expression marks slowly cycling 
      intestinal stem cells.
PG  - 179-84
LID - 10.1073/pnas.1013004108 [doi]
AB  - The intestinal epithelium is maintained by a population of rapidly cycling 
      (Lgr5(+)) intestinal stem cells (ISCs). It has been postulated, however, that 
      slowly cycling ISCs must also be present in the intestine to protect the genome 
      from accumulating deleterious mutations and to allow for a response to tissue 
      injury. Here, we identify a subpopulation of slowly cycling ISCs marked by mouse 
      telomerase reverse transcriptase (mTert) expression that can give rise to Lgr5(+) 
      cells. mTert-expressing cells distribute in a pattern along the crypt-villus axis 
      similar to long-term label-retaining cells (LRCs) and are resistant to tissue 
      injury. Lineage-tracing studies demonstrate that mTert(+) cells give rise to all 
      differentiated intestinal cell types, persist long term, and contribute to the 
      regenerative response following injury. Consistent with other highly regenerative 
      tissues, our results demonstrate that a slowly cycling stem cell population 
      exists within the intestine.
FAU - Montgomery, Robert K
AU  - Montgomery RK
AD  - Division of Gastroenterology, Children's Hospital Boston, Boston, MA 02115, USA.
FAU - Carlone, Diana L
AU  - Carlone DL
FAU - Richmond, Camilla A
AU  - Richmond CA
FAU - Farilla, Loredana
AU  - Farilla L
FAU - Kranendonk, Mariette E G
AU  - Kranendonk ME
FAU - Henderson, Daniel E
AU  - Henderson DE
FAU - Baffour-Awuah, Nana Yaa
AU  - Baffour-Awuah NY
FAU - Ambruzs, Dana M
AU  - Ambruzs DM
FAU - Fogli, Laura K
AU  - Fogli LK
FAU - Algra, Selma
AU  - Algra S
FAU - Breault, David T
AU  - Breault DT
LA  - eng
GR  - R01 DK 084056/DK/NIDDK NIH HHS/United States
GR  - R37 DK 32658/DK/NIDDK NIH HHS/United States
GR  - R01 DK084056/DK/NIDDK NIH HHS/United States
GR  - P30 HD 18655/HD/NICHD NIH HHS/United States
GR  - K08 DK066305/DK/NIDDK NIH HHS/United States
GR  - K08 DK 066305/DK/NIDDK NIH HHS/United States
GR  - R21 DK 078198/DK/NIDDK NIH HHS/United States
GR  - R37 DK032658/DK/NIDDK NIH HHS/United States
GR  - P30 HD018655/HD/NICHD NIH HHS/United States
GR  - P30 DK034854/DK/NIDDK NIH HHS/United States
GR  - P30 DK 34854/DK/NIDDK NIH HHS/United States
GR  - R21 DK078198/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101220
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Lgr5 protein, mouse)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.7.49 (Telomerase)
RN  - EC 2.7.7.49 (Tert protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Lineage/physiology
MH  - Flow Cytometry
MH  - Green Fluorescent Proteins/metabolism
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/*cytology
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Multipotent Stem Cells/cytology/*metabolism
MH  - Receptors, G-Protein-Coupled/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Telomerase/*metabolism
PMC - PMC3017192
COIS- The authors declare no conflict of interest.
EDAT- 2010/12/22 06:00
MHDA- 2011/02/11 06:00
PMCR- 2011/07/04
CRDT- 2010/12/22 06:00
PHST- 2010/12/22 06:00 [entrez]
PHST- 2010/12/22 06:00 [pubmed]
PHST- 2011/02/11 06:00 [medline]
PHST- 2011/07/04 00:00 [pmc-release]
AID - 1013004108 [pii]
AID - 201013004 [pii]
AID - 10.1073/pnas.1013004108 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):179-84. doi: 10.1073/pnas.1013004108. 
      Epub 2010 Dec 20.