PMID- 21220451
OWN - NLM
STAT- MEDLINE
DCOM- 20110214
LR  - 20220410
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 208
IP  - 1
DP  - 2011 Jan 17
TI  - Human B1 cells in umbilical cord and adult peripheral blood express the novel 
      phenotype CD20+ CD27+ CD43+ CD70-.
PG  - 67-80
LID - 10.1084/jem.20101499 [doi]
AB  - B1 cells differ in many ways from conventional B cells, most prominently in the 
      production of natural immunoglobulin, which is vitally important for protection 
      against pathogens. B1 cells have also been implicated in the pathogenesis of 
      autoimmune dyscrasias and malignant diseases. It has been impossible to 
      accurately study B1 cells during health and illness because the nature of human 
      B1 cells has not been successfully defined. This has produced controversy 
      regarding the existence of human B1 cells. Here, we determined the phenotype of 
      human B1 cells by testing sort-purified B cell fractions for three fundamental B1 
      cell functions based on mouse studies: spontaneous IgM secretion, efficient T 
      cell stimulation, and tonic intracellular signaling. We found that a small 
      population of CD20(+)CD27(+)CD43(+) cells present in both umbilical cord and 
      adult peripheral blood fulfilled these criteria and expressed a skewed B cell 
      receptor repertoire. These B cells express little or no surface CD69 and CD70, 
      both of which are markedly up-regulated after activation of CD20(+)CD27(-)CD43(-) 
      (naive) and CD20(+)CD27(+)CD43(-) (memory) B cells. This work identifies human B1 
      cells as CD20(+)CD27(+)CD43(+)CD70(-). We determined that the proportion of B1 
      cells declines with age, which may contribute to disease susceptibility. 
      Identification of human B1 cells provides a foundation for future studies on the 
      nature and role of these cells in human disease.
FAU - Griffin, Daniel O
AU  - Griffin DO
AD  - Elmezzi Graduate School of Molecular Medicine and Center and for Oncology and 
      Cell Biology, the Feinstein Institute for Medical Research, Manhasset, NY 11030, 
      USA.
FAU - Holodick, Nichol E
AU  - Holodick NE
FAU - Rothstein, Thomas L
AU  - Rothstein TL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110110
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens, CD)
RN  - 0 (Immunoglobulin M)
SB  - IM
EIN - J Exp Med. 2011 Apr 11;208(4):871
EIN - J Exp Med. 2011 Feb 14;208(2):409
EIN - J Exp Med. 2011 Jan 17;208(1):67
CIN - J Exp Med. 2011 Dec 19;208(13):2563-4. PMID: 22184680
CIN - J Exp Med. 2011 Dec 19;208(13):2565-6. PMID: 22184681
CIN - J Exp Med. 2012 Mar 12;209(3):433-4. PMID: 22412175
MH  - Adult
MH  - Antigens, CD/genetics/*immunology
MH  - B-Lymphocytes/*immunology/metabolism
MH  - Humans
MH  - Immunity, Innate
MH  - Immunoglobulin M/immunology
MH  - Immunologic Memory
MH  - Leukocytes/*immunology
MH  - Mutation
MH  - Phenotype
MH  - Signal Transduction
MH  - Umbilical Cord/*immunology/metabolism
PMC - PMC3023138
EDAT- 2011/01/12 06:00
MHDA- 2011/02/15 06:00
PMCR- 2011/07/17
CRDT- 2011/01/12 06:00
PHST- 2011/01/12 06:00 [entrez]
PHST- 2011/01/12 06:00 [pubmed]
PHST- 2011/02/15 06:00 [medline]
PHST- 2011/07/17 00:00 [pmc-release]
AID - jem.20101499 [pii]
AID - 20101499 [pii]
AID - 10.1084/jem.20101499 [doi]
PST - ppublish
SO  - J Exp Med. 2011 Jan 17;208(1):67-80. doi: 10.1084/jem.20101499. Epub 2011 Jan 10.