PMID- 21324352
OWN - NLM
STAT- MEDLINE
DCOM- 20110829
LR  - 20220223
IS  - 1090-2139 (Electronic)
IS  - 0889-1591 (Linking)
VI  - 25
IP  - 4
DP  - 2011 May
TI  - Minocycline reduces glioma expansion and invasion by attenuating microglial 
      MT1-MMP expression.
PG  - 624-8
LID - 10.1016/j.bbi.2011.01.015 [doi]
AB  - Glioma cells release soluble factors, which induce the expression of membrane 
      type 1 matrix metalloprotease (MT1-MMP) in tumor associated microglia and then 
      exploit MT1-MMP mediated matrix degradation for invasion. Here, we show that 
      minocycline blocked the increase in MT1-MMP expression and activity in cultivated 
      microglia stimulated with glioma conditioned medium. Glioma growth within an 
      organotypic brain slice preparation was reduced by minocycline and this reduction 
      depended on the presence of microglia. Glioma growth in an experimental mouse 
      model was strongly reduced by the addition of minocycline to drinking water, 
      compared to untreated controls. Coherently, we observed in our orthotopic glioma 
      implantation model, that MT1-MMP was abundantly expressed in glioma associated 
      microglia in controls, but was strongly attenuated in tumors of minocycline 
      treated animals. Overall, our study indicates that the clinically approved 
      antibiotic minocycline is a promising new candidate for adjuvant therapy against 
      malignant gliomas.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Markovic, D S
AU  - Markovic DS
AD  - Max Delbruck Center for Molecular Medicine, Cellular Neuroscience, Robert Rossle 
      Str. 10, 13125 Berlin, Germany.
FAU - Vinnakota, K
AU  - Vinnakota K
FAU - van Rooijen, N
AU  - van Rooijen N
FAU - Kiwit, J
AU  - Kiwit J
FAU - Synowitz, M
AU  - Synowitz M
FAU - Glass, R
AU  - Glass R
FAU - Kettenmann, H
AU  - Kettenmann H
LA  - eng
PT  - Journal Article
DEP - 20110213
PL  - Netherlands
TA  - Brain Behav Immun
JT  - Brain, behavior, and immunity
JID - 8800478
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Culture Media, Conditioned)
RN  - EC 3.4.24.80 (Matrix Metalloproteinase 14)
RN  - FYY3R43WGO (Minocycline)
SB  - IM
MH  - Animals
MH  - Antibiotics, Antineoplastic/*pharmacology
MH  - Brain Neoplasms/*drug therapy/enzymology
MH  - Cells, Cultured
MH  - Chemotherapy, Adjuvant
MH  - Culture Media, Conditioned
MH  - Glioma/*drug therapy/enzymology
MH  - Matrix Metalloproteinase 14/*metabolism
MH  - Mice
MH  - Microglia/cytology/*drug effects/enzymology
MH  - Minocycline/*pharmacology
MH  - Neoplasm Invasiveness/prevention & control
MH  - Neoplasms, Experimental
MH  - Organ Culture Techniques
EDAT- 2011/02/18 06:00
MHDA- 2011/08/30 06:00
CRDT- 2011/02/18 06:00
PHST- 2010/11/24 00:00 [received]
PHST- 2011/01/18 00:00 [revised]
PHST- 2011/01/25 00:00 [accepted]
PHST- 2011/02/18 06:00 [entrez]
PHST- 2011/02/18 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
AID - S0889-1591(11)00020-1 [pii]
AID - 10.1016/j.bbi.2011.01.015 [doi]
PST - ppublish
SO  - Brain Behav Immun. 2011 May;25(4):624-8. doi: 10.1016/j.bbi.2011.01.015. Epub 
      2011 Feb 13.