PMID- 21402776
OWN - NLM
STAT- MEDLINE
DCOM- 20110627
LR  - 20220330
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 31
IP  - 10
DP  - 2011 May
TI  - Essential and redundant functions of caudal family proteins in activating adult 
      intestinal genes.
PG  - 2026-39
LID - 10.1128/MCB.01250-10 [doi]
AB  - Transcription factors that potently induce cell fate often remain expressed in 
      the induced organ throughout life, but their requirements in adults are uncertain 
      and varied. Mechanistically, it is unclear if they activate only tissue-specific 
      genes or also directly repress heterologous genes. We conditionally inactivated 
      mouse Cdx2, a dominant regulator of intestinal development, and mapped its genome 
      occupancy in adult intestinal villi. Although homeotic transformation, observed 
      in Cdx2-null embryos, was absent in mutant adults, gene expression and cell 
      morphology were vitally compromised. Lethality was significantly accelerated in 
      mice lacking both Cdx2 and its homolog Cdx1, with particular exaggeration of 
      defects in villus enterocyte differentiation. Importantly, Cdx2 occupancy 
      correlated with hundreds of transcripts that fell but not with equal numbers that 
      rose with Cdx loss, indicating a predominantly activating role at intestinal 
      cis-regulatory regions. Integrated consideration of a transcription factor's 
      mutant phenotype and cistrome hence reveals the continued and distinct 
      requirement in adults of a critical developmental regulator that activates 
      tissue-specific genes.
FAU - Verzi, Michael P
AU  - Verzi MP
AD  - Department of Medical Oncology, Dana-Farber Cancer Institute, 44 Binney Street, 
      Boston, MA 02115, USA.
FAU - Shin, Hyunjin
AU  - Shin H
FAU - Ho, Li-Lun
AU  - Ho LL
FAU - Liu, X Shirley
AU  - Liu XS
FAU - Shivdasani, Ramesh A
AU  - Shivdasani RA
LA  - eng
SI  - GEO/GSE24633
GR  - 1K01DK088868-01/DK/NIDDK NIH HHS/United States
GR  - R01DK082889/DK/NIDDK NIH HHS/United States
GR  - K01 DK088868/DK/NIDDK NIH HHS/United States
GR  - R01HG4069/HG/NHGRI NIH HHS/United States
GR  - P50CA127003/CA/NCI NIH HHS/United States
GR  - R01 DK082889/DK/NIDDK NIH HHS/United States
GR  - R01 HG004069/HG/NHGRI NIH HHS/United States
GR  - P50 CA127003/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110314
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (CDX2 Transcription Factor)
RN  - 0 (Cdx1 protein, mouse)
RN  - 0 (Cdx2 protein, mouse)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - CDX2 Transcription Factor
MH  - Cell Differentiation
MH  - Enterocytes/*physiology
MH  - Gene Expression
MH  - Gene Expression Regulation, Developmental
MH  - Homeodomain Proteins/*genetics/metabolism
MH  - Intestinal Mucosa/embryology/growth & development/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Microarray Analysis
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/*genetics/metabolism
MH  - *Transcriptional Activation
PMC - PMC3133364
EDAT- 2011/03/16 06:00
MHDA- 2011/06/28 06:00
PMCR- 2011/11/01
CRDT- 2011/03/16 06:00
PHST- 2011/03/16 06:00 [entrez]
PHST- 2011/03/16 06:00 [pubmed]
PHST- 2011/06/28 06:00 [medline]
PHST- 2011/11/01 00:00 [pmc-release]
AID - MCB.01250-10 [pii]
AID - 1250-10 [pii]
AID - 10.1128/MCB.01250-10 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2011 May;31(10):2026-39. doi: 10.1128/MCB.01250-10. Epub 2011 Mar 
      14.