PMID- 21419747
OWN - NLM
STAT- MEDLINE
DCOM- 20111003
LR  - 20230108
IS  - 1875-9777 (Electronic)
IS  - 1875-9777 (Linking)
VI  - 8
IP  - 5
DP  - 2011 May 6
TI  - The intestinal stem cell signature identifies colorectal cancer stem cells and 
      predicts disease relapse.
PG  - 511-24
LID - 10.1016/j.stem.2011.02.020 [doi]
AB  - A frequent complication in colorectal cancer (CRC) is regeneration of the tumor 
      after therapy. Here, we report that a gene signature specific for adult 
      intestinal stem cells (ISCs) predicts disease relapse in CRC patients. ISCs are 
      marked by high expression of the EphB2 receptor, which becomes gradually silenced 
      as cells differentiate. Using EphB2 and the ISC marker Lgr5, we have 
      FACS-purified and profiled mouse ISCs, crypt proliferative progenitors, and late 
      transient amplifying cells to define a gene program specific for normal ISCs. 
      Furthermore, we discovered that ISC-specific genes identify a stem-like cell 
      population positioned at the bottom of tumor structures reminiscent of crypts. 
      EphB2 sorted ISC-like tumor cells display robust tumor-initiating capacity in 
      immunodeficient mice as well as long-term self-renewal potential. Taken together, 
      our data suggest that the ISC program defines a cancer stem cell niche within 
      colorectal tumors and plays a central role in CRC relapse.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Merlos-Suarez, Anna
AU  - Merlos-Suarez A
AD  - Oncology Program, Institute for Research in Biomedicine (IRB), Baldiri Reixac 10, 
      08028 Barcelona, Spain.
FAU - Barriga, Francisco M
AU  - Barriga FM
FAU - Jung, Peter
AU  - Jung P
FAU - Iglesias, Mar
AU  - Iglesias M
FAU - Cespedes, Maria Virtudes
AU  - Cespedes MV
FAU - Rossell, David
AU  - Rossell D
FAU - Sevillano, Marta
AU  - Sevillano M
FAU - Hernando-Momblona, Xavier
AU  - Hernando-Momblona X
FAU - da Silva-Diz, Victoria
AU  - da Silva-Diz V
FAU - Munoz, Purificacion
AU  - Munoz P
FAU - Clevers, Hans
AU  - Clevers H
FAU - Sancho, Elena
AU  - Sancho E
FAU - Mangues, Ramon
AU  - Mangues R
FAU - Batlle, Eduard
AU  - Batlle E
LA  - eng
SI  - GEO/GSE27605
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110317
PL  - United States
TA  - Cell Stem Cell
JT  - Cell stem cell
JID - 101311472
RN  - 0 (LGR5 protein, human)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - EC 2.7.10.1 (Receptor, EphB3)
SB  - IM
MH  - Adult Stem Cells/*metabolism/pathology
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Separation
MH  - Cell Surface Extensions/pathology
MH  - Cells, Cultured
MH  - Colonic Neoplasms/*diagnosis/pathology/physiopathology
MH  - Flow Cytometry
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - Intestines/*pathology
MH  - Mice
MH  - Mice, Knockout
MH  - Neoplasm Recurrence, Local
MH  - Neoplastic Stem Cells/*metabolism/pathology
MH  - Prognosis
MH  - Receptor, EphB3/genetics/*metabolism
MH  - Receptors, G-Protein-Coupled/metabolism
MH  - Stem Cell Niche
EDAT- 2011/03/23 06:00
MHDA- 2011/10/04 06:00
CRDT- 2011/03/23 06:00
PHST- 2010/04/26 00:00 [received]
PHST- 2011/01/10 00:00 [revised]
PHST- 2011/02/28 00:00 [accepted]
PHST- 2011/03/23 06:00 [entrez]
PHST- 2011/03/23 06:00 [pubmed]
PHST- 2011/10/04 06:00 [medline]
AID - S1934-5909(11)00110-X [pii]
AID - 10.1016/j.stem.2011.02.020 [doi]
PST - ppublish
SO  - Cell Stem Cell. 2011 May 6;8(5):511-24. doi: 10.1016/j.stem.2011.02.020. Epub 
      2011 Mar 17.