PMID- 21593866
OWN - NLM
STAT- MEDLINE
DCOM- 20110701
LR  - 20220331
IS  - 1476-4687 (Electronic)
IS  - 0028-0836 (Linking)
VI  - 473
IP  - 7347
DP  - 2011 May 19
TI  - Global quantification of mammalian gene expression control.
PG  - 337-42
LID - 10.1038/nature10098 [doi]
AB  - Gene expression is a multistep process that involves the transcription, 
      translation and turnover of messenger RNAs and proteins. Although it is one of 
      the most fundamental processes of life, the entire cascade has never been 
      quantified on a genome-wide scale. Here we simultaneously measured absolute mRNA 
      and protein abundance and turnover by parallel metabolic pulse labelling for more 
      than 5,000 genes in mammalian cells. Whereas mRNA and protein levels correlated 
      better than previously thought, corresponding half-lives showed no correlation. 
      Using a quantitative model we have obtained the first genome-scale prediction of 
      synthesis rates of mRNAs and proteins. We find that the cellular abundance of 
      proteins is predominantly controlled at the level of translation. Genes with 
      similar combinations of mRNA and protein stability shared functional properties, 
      indicating that half-lives evolved under energetic and dynamic constraints. 
      Quantitative information about all stages of gene expression provides a rich 
      resource and helps to provide a greater understanding of the underlying design 
      principles.
FAU - Schwanhausser, Bjorn
AU  - Schwanhausser B
AD  - Max Delbruck Center for Molecular Medicine, Robert-Rossle-Str. 10, D-13092 
      Berlin, Germany.
FAU - Busse, Dorothea
AU  - Busse D
FAU - Li, Na
AU  - Li N
FAU - Dittmar, Gunnar
AU  - Dittmar G
FAU - Schuchhardt, Johannes
AU  - Schuchhardt J
FAU - Wolf, Jana
AU  - Wolf J
FAU - Chen, Wei
AU  - Chen W
FAU - Selbach, Matthias
AU  - Selbach M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
CIN - Mol Syst Biol. 2011 May 24;7:498. PMID: 21613985
CIN - Nat Rev Genet. 2011 Aug;12(8):518. PMID: 21709688
CIN - Nat Methods. 2011 Jul;8(7):536. PMID: 21850735
EIN - Nature. 2013 Mar 7;495(7439):126-7. PMID: 23407496
MH  - Animals
MH  - Gene Expression Profiling/*methods
MH  - *Gene Expression Regulation
MH  - Half-Life
MH  - Mammals/genetics
MH  - Mice
MH  - Models, Genetic
MH  - NIH 3T3 Cells
MH  - Protein Biosynthesis/genetics
MH  - Proteins/*analysis/genetics/metabolism
MH  - RNA, Messenger/*analysis/biosynthesis/genetics/metabolism
MH  - Reproducibility of Results
MH  - Staining and Labeling
EDAT- 2011/05/20 06:00
MHDA- 2011/07/02 06:00
CRDT- 2011/05/20 06:00
PHST- 2010/11/16 00:00 [received]
PHST- 2011/04/01 00:00 [accepted]
PHST- 2011/05/20 06:00 [entrez]
PHST- 2011/05/20 06:00 [pubmed]
PHST- 2011/07/02 06:00 [medline]
AID - nature10098 [pii]
AID - 10.1038/nature10098 [doi]
PST - ppublish
SO  - Nature. 2011 May 19;473(7347):337-42. doi: 10.1038/nature10098.