PMID- 21598389
OWN - NLM
STAT- MEDLINE
DCOM- 20110927
LR  - 20110523
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 11
IP  - 11
DP  - 2011 Jun
TI  - Direct quantitation of MHC-bound peptide epitopes by selected reaction 
      monitoring.
PG  - 2336-40
LID - 10.1002/pmic.201000531 [doi]
AB  - We describe a cell-free approach that employs selected reaction monitoring (SRM) 
      in tandem mass spectrometry to identify and quantitate T-cell epitopes. This 
      approach utilises multiple epitope-specific SRM transitions to identify known 
      T-cell epitopes and an absolute quantitation (AQUA) peptide strategy to afford 
      AQUA. The advantage of a mass spectrometry-based approach over more traditional 
      cell-based assays resides in the robustness and transferability of an SRM 
      approach between laboratories and the ability of this strategy to detect multiple 
      peptides simultaneously without the requirement of epitope-specific reagents such 
      as T-cell lines. Thus, the SRM strategy for epitope quantitation will find 
      application in studies of antigen density, the link between epitope abundance and 
      immunogenicity, the dynamic range of epitope presentation and the abundance of 
      T-cell epitopes in disease.
CI  - Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Tan, Chor Teck
AU  - Tan CT
AD  - Department of Biochemistry and Molecular Biology, The Bio21 Molecular Science and 
      Biotechnology Institute, University of Melbourne, Melbourne, Victoria, Australia.
FAU - Croft, Nathan P
AU  - Croft NP
FAU - Dudek, Nadine L
AU  - Dudek NL
FAU - Williamson, Nicholas A
AU  - Williamson NA
FAU - Purcell, Anthony W
AU  - Purcell AW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110520
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (OVA-8)
RN  - 0 (Peptide Fragments)
RN  - 0 (Peptides)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cells, Cultured
MH  - Drug Discovery
MH  - Epitopes, T-Lymphocyte/*chemistry/metabolism
MH  - HLA Antigens/*analysis/chemistry
MH  - Mass Spectrometry/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Ovalbumin
MH  - Peptide Fragments
MH  - Peptides/chemistry/*immunology/metabolism
MH  - Protein Binding
MH  - Systems Biology
EDAT- 2011/05/21 06:00
MHDA- 2011/09/29 06:00
CRDT- 2011/05/21 06:00
PHST- 2010/08/23 00:00 [received]
PHST- 2011/01/17 00:00 [revised]
PHST- 2011/02/21 00:00 [accepted]
PHST- 2011/05/21 06:00 [entrez]
PHST- 2011/05/21 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - 10.1002/pmic.201000531 [doi]
PST - ppublish
SO  - Proteomics. 2011 Jun;11(11):2336-40. doi: 10.1002/pmic.201000531. Epub 2011 May 
      20.