PMID- 21606367
OWN - NLM
STAT- MEDLINE
DCOM- 20110826
LR  - 20220309
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 108
IP  - 23
DP  - 2011 Jun 7
TI  - Enhancer-driven membrane markers for analysis of nonautonomous mechanisms reveal 
      neuron-glia interactions in Drosophila.
PG  - 9673-8
LID - 10.1073/pnas.1106386108 [doi]
AB  - Extrinsic factors and the interactions of neurons with surrounding tissues are 
      essential for almost every aspect of neuronal development. Here we describe a 
      strategy of gene expression with an independent enhancer-driven cellular marker 
      (GEEM) for studying roles of cell-cell interactions and extrinsic factors in the 
      development of the Drosophila nervous system. Key to this strategy is robust 
      expression of enhancer-driven transgenic markers in specific neurons. To this 
      end, we have created vectors to achieve bright and even labeling of neuronal 
      processes, easy cloning of enhancer elements, and efficient and flexible 
      generation of transgenic animals. We provide examples of enhancer-driven membrane 
      markers for specific neurons in both the peripheral and central nervous systems 
      and their applications in the study of neuronal projections and connections in 
      the Drosophila brain. We further applied GEEM to examine the wrapping of sensory 
      neuron somas by glia during embryonic and larval stages, and neuron-glia 
      interaction during dendrite pruning in live animals, leading to the discovery 
      that glia play critical roles in the severing and degradation of proximal 
      dendrites. The GEEM paradigm should be applicable to the studies of both 
      cell-autonomous and nonautonomous regulations of any cell type.
FAU - Han, Chun
AU  - Han C
AD  - The Howard Hughes Medical Institute, Department of Physiology, University of 
      California, San Francisco, CA 94143-0725, USA.
FAU - Jan, Lily Yeh
AU  - Jan LY
FAU - Jan, Yuh-Nung
AU  - Jan YN
LA  - eng
GR  - R37 NS040929/NS/NINDS NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - 2R37NS040929/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110523
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Drosophila Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Brain/metabolism/physiology
MH  - Cell Communication/physiology
MH  - Central Nervous System/embryology/metabolism/physiology
MH  - Dendrites/metabolism/physiology
MH  - Drosophila/embryology/metabolism/*physiology
MH  - Drosophila Proteins/genetics/metabolism
MH  - Female
MH  - Green Fluorescent Proteins/genetics/*metabolism
MH  - Larva/metabolism/physiology
MH  - Male
MH  - Microscopy, Confocal
MH  - Neuroglia/cytology/metabolism/*physiology
MH  - Neurons/cytology/metabolism/*physiology
MH  - Neurophysiology/methods
MH  - Peripheral Nervous System/embryology/metabolism/physiology
MH  - Reproducibility of Results
PMC - PMC3111288
COIS- The authors declare no conflict of interest.
EDAT- 2011/05/25 06:00
MHDA- 2011/08/30 06:00
PMCR- 2011/05/23
CRDT- 2011/05/25 06:00
PHST- 2011/05/25 06:00 [entrez]
PHST- 2011/05/25 06:00 [pubmed]
PHST- 2011/08/30 06:00 [medline]
PHST- 2011/05/23 00:00 [pmc-release]
AID - 1106386108 [pii]
AID - 201106386 [pii]
AID - 10.1073/pnas.1106386108 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2011 Jun 7;108(23):9673-8. doi: 
      10.1073/pnas.1106386108. Epub 2011 May 23.