PMID- 2164210
OWN - NLM
STAT- MEDLINE
DCOM- 19900815
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 87
IP  - 13
DP  - 1990 Jul
TI  - Molecular cloning and expression of an additional epidermal growth factor 
      receptor-related gene.
PG  - 4905-9
AB  - Epidermal growth factor (EGF), transforming growth factor alpha (TGF-alpha), and 
      amphiregulin are structurally and functionally related growth regulatory 
      proteins. These secreted polypeptides all bind to the 170-kDa cell-surface EGF 
      receptor, activating its intrinsic kinase activity. However, amphiregulin 
      exhibits different activities than EGF and TGF-alpha in a number of biological 
      assays. Amphiregulin only partially competes with EGF for binding EGF receptor, 
      and amphiregulin does not induce anchorage-independent growth of normal rat 
      kidney cells (NRK) in the presence of TGF-beta. Amphiregulin also appears to 
      abrogate the stimulatory effect of TGF-alpha on the growth of several aggressive 
      epithelial carcinomas that overexpress EGF receptor. These findings suggest that 
      amphiregulin may interact with a separate receptor in certain cell types. Here we 
      report the cloning of another member of the human EGF receptor (HER) family of 
      receptor tyrosine kinases, which we have named "HER3/ERRB3." The cDNA was 
      isolated from a human carcinoma cell line, and its 6-kilobase transcript was 
      identified in various human tissues. We have generated peptide-specific antisera 
      that recognizes the 160-kDa HER3 protein when transiently expressed in COS cells. 
      These reagents will allow us to determine whether HER3 binds amphiregulin or 
      other growth regulatory proteins and what role HER3 protein plays in the 
      regulation of cell growth.
FAU - Plowman, G D
AU  - Plowman GD
AD  - Oncogen, Seattle, WA 98121.
FAU - Whitney, G S
AU  - Whitney GS
FAU - Neubauer, M G
AU  - Neubauer MG
FAU - Green, J M
AU  - Green JM
FAU - McDonald, V L
AU  - McDonald VL
FAU - Todaro, G J
AU  - Todaro GJ
FAU - Shoyab, M
AU  - Shoyab M
LA  - eng
SI  - GENBANK/M34309
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Oligonucleotide Probes)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular/methods
MH  - ErbB Receptors/*genetics
MH  - *Gene Expression
MH  - *Genes
MH  - Humans
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Protein Conformation
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Transfection
MH  - Tumor Cells, Cultured/enzymology
PMC - PMC54229
EDAT- 1990/07/01 00:00
MHDA- 1990/07/01 00:01
PMCR- 1991/01/01
CRDT- 1990/07/01 00:00
PHST- 1990/07/01 00:00 [pubmed]
PHST- 1990/07/01 00:01 [medline]
PHST- 1990/07/01 00:00 [entrez]
PHST- 1991/01/01 00:00 [pmc-release]
AID - 10.1073/pnas.87.13.4905 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1990 Jul;87(13):4905-9. doi: 10.1073/pnas.87.13.4905.