PMID- 21702370
OWN - NLM
STAT- MEDLINE
DCOM- 20110802
LR  - 20221207
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 7
IP  - 2
DP  - 2011 Apr
TI  - Anti-angiogenic effects of betulinic acid administered in nanoemulsion 
      formulation using chorioallantoic membrane assay.
PG  - 317-24
AB  - Betulinic acid (3beta, hydroxy-lup-20(29)-en-28-oic acid, BA), a pentacyclic 
      triterpenoid, is derived from a widely distributed natural anticancer compound 
      betulin. It has selective anticancer activity against several tumor cells, and 
      recently it was shown that it also possess anti-angiogenic effects. The objective 
      of this study was to formulate betulinic acid, a poorly aqueous-soluble compound, 
      in flax-seed oil containing nanoemulsion formulation for enhanced delivery 
      efficiency and to effectively inhibit the tumor angiogenic process. The 
      nanoemulsion was prepared using high pressure homogenization method with a 
      Microfludizer processor. The betulinic acid nanoemulsion was studied for the 
      effect on the angiogenic process by performing the in vivo chick embryo 
      chorioallantoic membrane (CAM) assay. The sample volume of 1 microl and 5 microl 
      of the blank and BA nanoemulsions were applied directly on the CAM. The 
      preliminary results from macroscopic, morphological and immunohistochemical 
      evaluations have shown that morphological change was produced in the CAM 
      mesenchyme with a negative impact on the normal growth of the capillaries. 
      Betulinic acid does possess anti-angiogenic activity in a dose dependent manner, 
      and the nanoemulsion formulation maintained this effect.
FAU - Dehelean, Cristina A
AU  - Dehelean CA
AD  - Toxicology Department, University of Medicine and Pharmacy Victor Babes 
      Timisoara, Eftimie Murgu Square, No. 2, Timisoara, 300041, Romania.
FAU - Feflea, Stefana
AU  - Feflea S
FAU - Ganta, Srinivas
AU  - Ganta S
FAU - Amiji, Mansoor
AU  - Amiji M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 0 (Angiogenesis Inhibitors)
RN  - 0 (Emulsions)
RN  - 0 (Pentacyclic Triterpenes)
RN  - 0 (Triterpenes)
RN  - 4G6A18707N (Betulinic Acid)
SB  - IM
MH  - Angiogenesis Inhibitors/administration & dosage/chemistry/*pharmacology
MH  - Animals
MH  - Chemistry, Pharmaceutical
MH  - Chick Embryo
MH  - Chorioallantoic Membrane/*blood supply/cytology/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Emulsions/administration & dosage/chemistry
MH  - Immunohistochemistry
MH  - Microscopy, Electron, Transmission
MH  - Nanostructures/*administration & dosage/chemistry
MH  - Neovascularization, Physiologic/drug effects
MH  - Pentacyclic Triterpenes
MH  - Solubility
MH  - Triterpenes/administration & dosage/chemistry/*pharmacology
MH  - Betulinic Acid
EDAT- 2011/06/28 06:00
MHDA- 2011/08/04 06:00
CRDT- 2011/06/28 06:00
PHST- 2011/06/28 06:00 [entrez]
PHST- 2011/06/28 06:00 [pubmed]
PHST- 2011/08/04 06:00 [medline]
AID - 10.1166/jbn.2011.1297 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2011 Apr;7(2):317-24. doi: 10.1166/jbn.2011.1297.