PMID- 21892181
OWN - NLM
STAT- MEDLINE
DCOM- 20111207
LR  - 20220408
IS  - 1546-170X (Electronic)
IS  - 1078-8956 (Linking)
VI  - 17
IP  - 10
DP  - 2011 Sep 4
TI  - Isolation and in vitro expansion of human colonic stem cells.
PG  - 1225-7
LID - 10.1038/nm.2470 [doi]
AB  - Here we describe the isolation of stem cells of the human colonic epithelium. 
      Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows 
      the purification of different cell types from human colon mucosa biopsies. The 
      highest EPHB2 surface levels correspond to epithelial colonic cells with the 
      longest telomeres and elevated expression of intestinal stem cell (ISC) marker 
      genes. Moreover, using culturing conditions that recreate the ISC niche, a 
      substantial proportion of EPHB2-high cells can be expanded in vitro as an 
      undifferentiated and multipotent population.
FAU - Jung, Peter
AU  - Jung P
AD  - Oncology program, Institute for Research in Biomedicine, Barcelona, Spain.
FAU - Sato, Toshiro
AU  - Sato T
FAU - Merlos-Suarez, Anna
AU  - Merlos-Suarez A
FAU - Barriga, Francisco M
AU  - Barriga FM
FAU - Iglesias, Mar
AU  - Iglesias M
FAU - Rossell, David
AU  - Rossell D
FAU - Auer, Herbert
AU  - Auer H
FAU - Gallardo, Mercedes
AU  - Gallardo M
FAU - Blasco, Maria A
AU  - Blasco MA
FAU - Sancho, Elena
AU  - Sancho E
FAU - Clevers, Hans
AU  - Clevers H
FAU - Batlle, Eduard
AU  - Batlle E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110904
PL  - United States
TA  - Nat Med
JT  - Nature medicine
JID - 9502015
RN  - EC 2.7.10.1 (Receptor, EphB2)
SB  - IM
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation
MH  - Colon/*cytology
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/*cytology
MH  - Microscopy, Confocal
MH  - Multipotent Stem Cells/cytology/*physiology
MH  - Real-Time Polymerase Chain Reaction
MH  - Receptor, EphB2/*metabolism
MH  - Telomere/metabolism
EDAT- 2011/09/06 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/09/06 06:00
PHST- 2011/02/25 00:00 [received]
PHST- 2011/08/14 00:00 [accepted]
PHST- 2011/09/06 06:00 [entrez]
PHST- 2011/09/06 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - nm.2470 [pii]
AID - 10.1038/nm.2470 [doi]
PST - epublish
SO  - Nat Med. 2011 Sep 4;17(10):1225-7. doi: 10.1038/nm.2470.