PMID- 21949686
OWN - NLM
STAT- MEDLINE
DCOM- 20120301
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 9
DP  - 2011
TI  - PS integrins and laminins: key regulators of cell migration during Drosophila 
      embryogenesis.
PG  - e23893
LID - 10.1371/journal.pone.0023893 [doi]
LID - e23893
AB  - During embryonic development, there are numerous cases where organ or tissue 
      formation depends upon the migration of primordial cells. In the Drosophila 
      embryo, the visceral mesoderm (vm) acts as a substrate for the migration of 
      several cell populations of epithelial origin, including the endoderm, the 
      trachea and the salivary glands. These migratory processes require both integrins 
      and laminins. The current model is that alphaPS1betaPS (PS1) and/or alphaPS3betaPS (PS3) 
      integrins are required in migrating cells, whereas alphaPS2betaPS (PS2) integrin is 
      required in the vm, where it performs an as yet unidentified function. Here, we 
      show that PS1 integrins are also required for the migration over the vm of cells 
      of mesodermal origin, the caudal visceral mesoderm (CVM). These results support a 
      model in which PS1 might have evolved to acquire the migratory function of 
      integrins, irrespective of the origin of the tissue. This integrin function is 
      highly specific and its specificity resides mainly in the extracellular domain. 
      In addition, we have identified the Laminin alpha1,2 trimer, as the key extracellular 
      matrix (ECM) component regulating CVM migration. Furthermore, we show that, as it 
      is the case in vertebrates, integrins, and specifically PS2, contributes to CVM 
      movement by participating in the correct assembly of the ECM that serves as 
      tracks for migration.
FAU - Urbano, Jose M
AU  - Urbano JM
AD  - Centro Andaluz de Biologia del Desarrollo, (CSIC)-Universidad Pablo de Olavide, 
      Sevilla, Spain.
FAU - Dominguez-Gimenez, Paloma
AU  - Dominguez-Gimenez P
FAU - Estrada, Beatriz
AU  - Estrada B
FAU - Martin-Bermudo, Maria D
AU  - Martin-Bermudo MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110916
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Drosophila Proteins)
RN  - 0 (Integrins)
RN  - 0 (Laminin)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - *Cell Movement
MH  - Drosophila/embryology/genetics/metabolism
MH  - Drosophila Proteins/genetics/*metabolism
MH  - Embryo, Nonmammalian/cytology/embryology/metabolism
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - In Situ Hybridization
MH  - Integrins/genetics/*metabolism
MH  - Laminin/genetics/*metabolism
MH  - Mesoderm/cytology/embryology/metabolism
MH  - Microscopy, Confocal
MH  - Time-Lapse Imaging
PMC - PMC3174947
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/09/29 06:00
MHDA- 2012/03/02 06:00
PMCR- 2011/09/16
CRDT- 2011/09/28 06:00
PHST- 2011/06/13 00:00 [received]
PHST- 2011/07/27 00:00 [accepted]
PHST- 2011/09/28 06:00 [entrez]
PHST- 2011/09/29 06:00 [pubmed]
PHST- 2012/03/02 06:00 [medline]
PHST- 2011/09/16 00:00 [pmc-release]
AID - PONE-D-11-10688 [pii]
AID - 10.1371/journal.pone.0023893 [doi]
PST - ppublish
SO  - PLoS One. 2011;6(9):e23893. doi: 10.1371/journal.pone.0023893. Epub 2011 Sep 16.