PMID- 21962510
OWN - NLM
STAT- MEDLINE
DCOM- 20111130
LR  - 20250103
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 147
IP  - 1
DP  - 2011 Sep 30
TI  - Translocation-capture sequencing reveals the extent and nature of chromosomal 
      rearrangements in B lymphocytes.
PG  - 95-106
LID - 10.1016/j.cell.2011.07.048 [doi]
AB  - Chromosomal rearrangements, including translocations, require formation and 
      joining of DNA double strand breaks (DSBs). These events disrupt the integrity of 
      the genome and are frequently involved in producing leukemias, lymphomas and 
      sarcomas. Despite the importance of these events, current understanding of their 
      genesis is limited. To examine the origins of chromosomal rearrangements we 
      developed Translocation Capture Sequencing (TC-Seq), a method to document 
      chromosomal rearrangements genome-wide, in primary cells. We examined over 
      180,000 rearrangements obtained from 400 million B lymphocytes, revealing that 
      proximity between DSBs, transcriptional activity and chromosome territories are 
      key determinants of genome rearrangement. Specifically, rearrangements tend to 
      occur in cis and to transcribed genes. Finally, we find that activation-induced 
      cytidine deaminase (AID) induces the rearrangement of many genes found as 
      translocation partners in mature B cell lymphoma.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Klein, Isaac A
AU  - Klein IA
AD  - Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 
      10065, USA.
FAU - Resch, Wolfgang
AU  - Resch W
FAU - Jankovic, Mila
AU  - Jankovic M
FAU - Oliveira, Thiago
AU  - Oliveira T
FAU - Yamane, Arito
AU  - Yamane A
FAU - Nakahashi, Hirotaka
AU  - Nakahashi H
FAU - Di Virgilio, Michela
AU  - Di Virgilio M
FAU - Bothmer, Anne
AU  - Bothmer A
FAU - Nussenzweig, Andre
AU  - Nussenzweig A
FAU - Robbiani, Davide F
AU  - Robbiani DF
FAU - Casellas, Rafael
AU  - Casellas R
FAU - Nussenzweig, Michel C
AU  - Nussenzweig MC
LA  - eng
GR  - R01 AI037526/AI/NIAID NIH HHS/United States
GR  - GM07739/GM/NIGMS NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R01 AI037526-17/AI/NIAID NIH HHS/United States
GR  - ImNIH/Intramural NIH HHS/United States
GR  - T32 GM007739/GM/NIGMS NIH HHS/United States
GR  - AI037526/AI/NIAID NIH HHS/United States
GR  - R37 AI037526/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - EC 3.5.4.- (AICDA (Activation-Induced Cytidine Deaminase))
RN  - EC 3.5.4.5 (Cytidine Deaminase)
SB  - IM
CIN - Cell. 2011 Sep 30;147(1):20-2. doi: 10.1016/j.cell.2011.09.005. PMID: 21962501
CIN - Nat Rev Genet. 2011 Oct 18;12(11):741. doi: 10.1038/nrg3090. PMID: 22005978
MH  - Animals
MH  - B-Lymphocytes/*metabolism
MH  - Cells, Cultured
MH  - Cytidine Deaminase/metabolism
MH  - Genes, myc
MH  - *Genome
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Mice
MH  - *Mutagenesis
MH  - Neoplasms/genetics
MH  - Sequence Analysis, DNA/methods
MH  - Spleen/cytology
MH  - *Translocation, Genetic
MH  - AICDA (Activation-Induced Cytidine Deaminase)
PMC - PMC3190307
MID - NIHMS322290
EDAT- 2011/10/04 06:00
MHDA- 2011/12/13 00:00
PMCR- 2012/09/30
CRDT- 2011/10/04 06:00
PHST- 2011/05/24 00:00 [received]
PHST- 2011/07/14 00:00 [revised]
PHST- 2011/07/27 00:00 [accepted]
PHST- 2011/10/04 06:00 [entrez]
PHST- 2011/10/04 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2012/09/30 00:00 [pmc-release]
AID - S0092-8674(11)00999-8 [pii]
AID - 10.1016/j.cell.2011.07.048 [doi]
PST - ppublish
SO  - Cell. 2011 Sep 30;147(1):95-106. doi: 10.1016/j.cell.2011.07.048.