PMID- 22075147 OWN - NLM STAT- MEDLINE DCOM- 20120201 LR - 20211203 IS - 1878-1551 (Electronic) IS - 1534-5807 (Print) IS - 1534-5807 (Linking) VI - 21 IP - 5 DP - 2011 Nov 15 TI - Tao-1 phosphorylates Hippo/MST kinases to regulate the Hippo-Salvador-Warts tumor suppressor pathway. PG - 888-95 LID - 10.1016/j.devcel.2011.08.028 [doi] AB - Recent studies have shown that the Hippo-Salvador-Warts (HSW) pathway restrains tissue growth by phosphorylating and inactivating the oncoprotein Yorkie. How growth-suppressive signals are transduced upstream of Hippo remains unclear. We show that the Sterile 20 family kinase, Tao-1, directly phosphorylates T195 in the Hippo activation loop and that, like other HSW pathway genes, Tao-1 functions to restrict cell proliferation in developing imaginal epithelia. This relationship appears to be evolutionarily conserved, because mammalian Tao-1 similarly affects MST kinases. In S2 cells, Tao-1 mediates the effects of the upstream HSW components Merlin and Expanded, consistent with the idea that Tao-1 functions in tissues to regulate Hippo phosphorylation. These results demonstrate that one family of Ste20 kinases can activate another and identify Tao-1 as a component of the regulatory network controlling HSW pathway signaling, and therefore tissue growth, during development. CI - Copyright (c) 2011 Elsevier Inc. All rights reserved. FAU - Boggiano, Julian C AU - Boggiano JC AD - Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL 60637, USA. FAU - Vanderzalm, Pamela J AU - Vanderzalm PJ FAU - Fehon, Richard G AU - Fehon RG LA - eng GR - T32 CA009594/CA/NCI NIH HHS/United States GR - T32 HD055164/HD/NICHD NIH HHS/United States GR - R01 NS034783-15/NS/NINDS NIH HHS/United States GR - NS034783/NS/NINDS NIH HHS/United States GR - R01 NS034783/NS/NINDS NIH HHS/United States GR - T32 GM007197/GM/NIGMS NIH HHS/United States GR - T32 GMO7197/PHS HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PL - United States TA - Dev Cell JT - Developmental cell JID - 101120028 RN - 0 (Cell Cycle Proteins) RN - 0 (Drosophila Proteins) RN - 0 (Intracellular Signaling Peptides and Proteins) RN - 0 (Tumor Suppressor Proteins) RN - 0 (sav protein, Drosophila) RN - EC 2.7.- (Protein Kinases) RN - EC 2.7.1.- (wts protein, Drosophila) RN - EC 2.7.11.1 (Protein Serine-Threonine Kinases) RN - EC 2.7.11.1 (TAO1 protein kinase) RN - EC 2.7.11.1 (hpo protein, Drosophila) RN - EC 2.7.11.25 (MAP Kinase Kinase Kinases) SB - IM MH - Animals MH - Cell Cycle Proteins/*metabolism MH - Cells, Cultured MH - Drosophila Proteins/*metabolism MH - Drosophila melanogaster/enzymology/genetics/*metabolism MH - Intracellular Signaling Peptides and Proteins/*metabolism MH - MAP Kinase Kinase Kinases/*metabolism MH - Phosphorylation MH - Protein Kinases/*metabolism MH - Protein Serine-Threonine Kinases/*metabolism MH - Tumor Suppressor Proteins/*metabolism PMC - PMC3217187 MID - NIHMS322922 EDAT- 2011/11/15 06:00 MHDA- 2012/02/02 06:00 PMCR- 2012/11/15 CRDT- 2011/11/15 06:00 PHST- 2011/04/16 00:00 [received] PHST- 2011/07/11 00:00 [revised] PHST- 2011/08/29 00:00 [accepted] PHST- 2011/11/15 06:00 [entrez] PHST- 2011/11/15 06:00 [pubmed] PHST- 2012/02/02 06:00 [medline] PHST- 2012/11/15 00:00 [pmc-release] AID - S1534-5807(11)00366-2 [pii] AID - 10.1016/j.devcel.2011.08.028 [doi] PST - ppublish SO - Dev Cell. 2011 Nov 15;21(5):888-95. doi: 10.1016/j.devcel.2011.08.028.