PMID- 22201549
OWN - NLM
STAT- MEDLINE
DCOM- 20120419
LR  - 20211021
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 234
IP  - 1
DP  - 2012 Mar
TI  - Effects of aging on blood brain barrier and matrix metalloproteases following 
      controlled cortical impact in mice.
PG  - 50-61
LID - 10.1016/j.expneurol.2011.12.016 [doi]
AB  - Aging alters the ability of the brain to respond to injury. One of the major 
      differences between the adult and aged brain is that comparable injuries lead to 
      greater blood brain barrier disruption in the aged brain. The goals of these 
      studies were to quantify the effects of age on BBB permeability using high field 
      strength MRI T1 mapping and to determine whether activation of matrix 
      metalloproteases, their inhibitors, or expression of blood brain barrier 
      structural proteins, occludin, zonnula occludins-1 (ZO-1) and claudin-5 were 
      altered following injury to the aged C57/BL6 mouse brain. T1 mapping studies 
      revealed greater blood brain barrier permeability in the aged (21-24 months old) 
      brain than in the adult (4-6 months old) following controlled cortical impact. 
      The increased blood brain barrier permeability in the pericontusional region was 
      confirmed with IgG immunohistochemistry. MMP-9 activity was increased following 
      controlled cortical impact in the aged brain, and this was accompanied by 
      increased MMP-9 gene expression. MMP-2 activity was higher in the uninjured aged 
      brain than in the adult brain. Occludin and ZO-1 mRNA levels were unchanged 
      following injury in either age group, but claudin-5 mRNA levels were lower in the 
      aged than the adult brain following injury. These results demonstrate 
      quantitative increases in blood brain barrier permeability in the aged brain 
      following injury that are accompanied by increased MMP-9 activation and decreased 
      blood brain barrier repair responses.
CI  - Copyright A(c) 2011. Published by Elsevier Inc.
FAU - Lee, Phil
AU  - Lee P
AD  - Hoglund Brain Imaging Center, University of Kansas Medical Center, 3901 Rainbow 
      Blvd., Kansas City, KS 66160, USA.
FAU - Kim, Jieun
AU  - Kim J
FAU - Williams, Rachel
AU  - Williams R
FAU - Sandhir, Rajat
AU  - Sandhir R
FAU - Gregory, Eugene
AU  - Gregory E
FAU - Brooks, William M
AU  - Brooks WM
FAU - Berman, Nancy E J
AU  - Berman NE
LA  - eng
GR  - P20 RR016475/RR/NCRR NIH HHS/United States
GR  - P30 AG035982/AG/NIA NIH HHS/United States
GR  - P30 HD02528/HD/NICHD NIH HHS/United States
GR  - R01 AG031140/AG/NIA NIH HHS/United States
GR  - R01AG31140/AG/NIA NIH HHS/United States
GR  - P30 HD002528/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20111219
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Membrane Proteins)
RN  - 0 (Occludin)
RN  - 0 (Ocln protein, mouse)
RN  - 0 (Phosphoproteins)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (Tjp1 protein, mouse)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - K2I13DR72L (Gadolinium DTPA)
SB  - IM
MH  - *Aging
MH  - Animals
MH  - Blood-Brain Barrier/*physiopathology
MH  - Brain Injuries/*enzymology/*pathology
MH  - Brain Mapping
MH  - Cerebral Cortex/*pathology
MH  - Disease Models, Animal
MH  - Gadolinium DTPA
MH  - Gene Expression Regulation/physiology
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Occludin
MH  - Permeability
MH  - Phosphoproteins/metabolism
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinases/metabolism
MH  - Trauma Severity Indices
MH  - Zonula Occludens-1 Protein
PMC - PMC4042317
MID - NIHMS584356
EDAT- 2011/12/29 06:00
MHDA- 2012/04/20 06:00
PMCR- 2014/06/03
CRDT- 2011/12/29 06:00
PHST- 2011/10/10 00:00 [received]
PHST- 2011/12/05 00:00 [revised]
PHST- 2011/12/09 00:00 [accepted]
PHST- 2011/12/29 06:00 [entrez]
PHST- 2011/12/29 06:00 [pubmed]
PHST- 2012/04/20 06:00 [medline]
PHST- 2014/06/03 00:00 [pmc-release]
AID - S0014-4886(11)00468-7 [pii]
AID - 10.1016/j.expneurol.2011.12.016 [doi]
PST - ppublish
SO  - Exp Neurol. 2012 Mar;234(1):50-61. doi: 10.1016/j.expneurol.2011.12.016. Epub 
      2011 Dec 19.