PMID- 22308434
OWN - NLM
STAT- MEDLINE
DCOM- 20120330
LR  - 20211021
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 109
IP  - 6
DP  - 2012 Feb 7
TI  - Ecotopic viral integration site 1 (EVI1) regulates multiple cellular processes 
      important for cancer and is a synergistic partner for FOS protein in invasive 
      tumors.
PG  - 2168-73
LID - 10.1073/pnas.1119229109 [doi]
AB  - Ecotropic viral integration site 1 (EVI1) is an oncogenic dual domain zinc finger 
      transcription factor that plays an essential role in the regulation of 
      hematopoietic stem cell renewal, and its overexpression in myeloid leukemia and 
      epithelial cancers is associated with poor patient survival. Despite the 
      discovery of EVI1 in 1988 and its emerging role as a dominant oncogene in various 
      types of cancer, few EVI1 target genes are known. This lack of knowledge has 
      precluded a clear understanding of exactly how EVI1 contributes to cancer. Using 
      a combination of ChIP-Seq and microarray studies in human ovarian carcinoma 
      cells, we show that the two zinc finger domains of EVI1 bind to DNA independently 
      and regulate different sets of target genes. Strikingly, an enriched fraction of 
      EVI1 target genes are cancer genes or genes associated with cancer. We also show 
      that more than 25% of EVI1-occupied genes contain linked EVI1 and activator 
      protein (AP)1 DNA binding sites, and this finding provides evidence for a 
      synergistic cooperative interaction between EVI1 and the AP1 family member FOS in 
      the regulation of cell adhesion, proliferation, and colony formation. An 
      increased number of dual EVI1/AP1 target genes are also differentially regulated 
      in late-stage ovarian carcinomas, further confirming the importance of the 
      functional cooperation between EVI1 and FOS. Collectively, our data indicate that 
      EVI1 is a multipurpose transcription factor that synergizes with FOS in invasive 
      tumors.
FAU - Bard-Chapeau, Emilie A
AU  - Bard-Chapeau EA
AD  - Institute of Molecular and Cell Biology, Singapore 138673.
FAU - Jeyakani, Justin
AU  - Jeyakani J
FAU - Kok, Chung H
AU  - Kok CH
FAU - Muller, Julius
AU  - Muller J
FAU - Chua, Belinda Q
AU  - Chua BQ
FAU - Gunaratne, Jayantha
AU  - Gunaratne J
FAU - Batagov, Arsen
AU  - Batagov A
FAU - Jenjaroenpun, Piroon
AU  - Jenjaroenpun P
FAU - Kuznetsov, Vladimir A
AU  - Kuznetsov VA
FAU - Wei, Chia-Lin
AU  - Wei CL
FAU - D'Andrea, Richard J
AU  - D'Andrea RJ
FAU - Bourque, Guillaume
AU  - Bourque G
FAU - Jenkins, Nancy A
AU  - Jenkins NA
FAU - Copeland, Neal G
AU  - Copeland NG
LA  - eng
SI  - GEO/GSE25210
SI  - GEO/GSE25212
SI  - GEO/GSE25213
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120119
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MDS1 and EVI1 Complex Locus Protein)
RN  - 0 (MECOM protein, human)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Binding Sites
MH  - Cell Adhesion
MH  - Chromatin Immunoprecipitation
MH  - DNA/genetics/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Epithelial Cells/metabolism/pathology
MH  - Gene Expression Regulation
MH  - HeLa Cells
MH  - Humans
MH  - MDS1 and EVI1 Complex Locus Protein
MH  - Neoplasm Invasiveness
MH  - Neoplasms/*metabolism/*pathology
MH  - Protein Binding
MH  - Proto-Oncogene Proteins c-fos/*metabolism
MH  - Proto-Oncogenes
MH  - Transcription Factors/*metabolism
PMC - PMC3277513
COIS- The authors declare no conflict of interest.
EDAT- 2012/02/07 06:00
MHDA- 2012/03/31 06:00
PMCR- 2012/08/07
CRDT- 2012/02/07 06:00
PHST- 2012/02/07 06:00 [entrez]
PHST- 2012/02/07 06:00 [pubmed]
PHST- 2012/03/31 06:00 [medline]
PHST- 2012/08/07 00:00 [pmc-release]
AID - 1119229109 [pii]
AID - 201119229 [pii]
AID - 10.1073/pnas.1119229109 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2012 Feb 7;109(6):2168-73. doi: 
      10.1073/pnas.1119229109. Epub 2012 Jan 19.