PMID- 22652549
OWN - NLM
STAT- MEDLINE
DCOM- 20130108
LR  - 20220408
IS  - 1873-4995 (Electronic)
IS  - 0168-3659 (Linking)
VI  - 161
IP  - 3
DP  - 2012 Aug 10
TI  - Carbosilane dendrimer nanotechnology outlines of the broad HIV blocker profile.
PG  - 949-58
LID - 10.1016/j.jconrel.2012.04.050 [doi]
AB  - Researchers have been working hard for more than 20 years to develop safe and 
      effective microbicides to empower women to better control their own sexual life 
      and to protect themselves against HIV and other sexually transmitted infections 
      (STIs). Microbicide classes include moderately specific macromolecular anionic 
      polymers that block HIV and other STIs, and HIV specific drugs that inhibit viral 
      entry and reverse transcription. Based on innovative nanotechnology design, we 
      showed a novel water-soluble anionic carbosilane dendrimer (2G-S16) as a 
      propitious molecule against HIV-infection. A state-of-the-art research was 
      accomplished that focused on biomedical cutting-edge techniques such as in vitro 
      and in vivo cytotoxicity assays performed on female rabbit genital tracts, 
      simulate in vitro model of vaginal epithelium in order to evaluate HIV 
      transmission blockade through the monolayer, complete gene expression profiling 
      experiment to study deregulated genes after 2G-S16 exposition, molecular dynamics 
      simulation of 2G-S16 molecule against principal proteins of HIV particles and 
      pro- and anti-inflammatory cytokine profile study. Therefore, a high-throughput 
      study and detailed analysis of the results were achieved in this article. We 
      provided promising outcomes to encourage 2G-S16 as a hopeful microbicide.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Chonco, L
AU  - Chonco L
AD  - Laboratorio de InmunoBiologia Molecular y Plataforma de Laboratorio, Hospital 
      General Universitario Gregorio Maranon, Madrid, Spain.
FAU - Pion, M
AU  - Pion M
FAU - Vacas, E
AU  - Vacas E
FAU - Rasines, B
AU  - Rasines B
FAU - Maly, M
AU  - Maly M
FAU - Serramia, M J
AU  - Serramia MJ
FAU - Lopez-Fernandez, L
AU  - Lopez-Fernandez L
FAU - De la Mata, J
AU  - De la Mata J
FAU - Alvarez, S
AU  - Alvarez S
FAU - Gomez, R
AU  - Gomez R
FAU - Munoz-Fernandez, M A
AU  - Munoz-Fernandez MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20120528
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Cytokines)
RN  - 0 (Dendrimers)
RN  - 0 (Silanes)
RN  - 0 (carbosilane)
SB  - IM
MH  - Administration, Topical
MH  - Animals
MH  - Anti-Infective Agents/*administration & dosage
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendrimers/*administration & dosage
MH  - Female
MH  - Gene Expression Profiling
MH  - HIV Infections/prevention & control
MH  - HIV-1/*drug effects/physiology
MH  - HIV-2/*drug effects/physiology
MH  - Humans
MH  - Leukocytes, Mononuclear
MH  - Nanotechnology
MH  - Rabbits
MH  - Silanes/*administration & dosage
MH  - Vagina
MH  - Virus Replication/drug effects
EDAT- 2012/06/02 06:00
MHDA- 2013/01/09 06:00
CRDT- 2012/06/02 06:00
PHST- 2012/03/31 00:00 [received]
PHST- 2012/04/26 00:00 [accepted]
PHST- 2012/06/02 06:00 [entrez]
PHST- 2012/06/02 06:00 [pubmed]
PHST- 2013/01/09 06:00 [medline]
AID - S0168-3659(12)00443-9 [pii]
AID - 10.1016/j.jconrel.2012.04.050 [doi]
PST - ppublish
SO  - J Control Release. 2012 Aug 10;161(3):949-58. doi: 10.1016/j.jconrel.2012.04.050. 
      Epub 2012 May 28.