PMID- 22696213
OWN - NLM
STAT- MEDLINE
DCOM- 20121105
LR  - 20220129
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 287
IP  - 32
DP  - 2012 Aug 3
TI  - Rad5-dependent DNA repair functions of the Saccharomyces cerevisiae FANCM protein 
      homolog Mph1.
PG  - 26563-75
LID - 10.1074/jbc.M112.369918 [doi]
AB  - Interstrand cross-links (ICLs) covalently link complementary DNA strands, block 
      DNA replication, and transcription and must be removed to allow cell survival. 
      Several pathways, including the Fanconi anemia (FA) pathway, can faithfully 
      repair ICLs and maintain genomic integrity; however, the precise mechanisms of 
      most ICL repair processes remain enigmatic. In this study we genetically 
      characterized a conserved yeast ICL repair pathway composed of the yeast homologs 
      (Mph1, Chl1, Mhf1, Mhf2) of four FA proteins (FANCM, FANCJ, MHF1, MHF2). This 
      pathway is epistatic with Rad5-mediated DNA damage bypass and distinct from the 
      ICL repair pathways mediated by Rad18 and Pso2. In addition, consistent with the 
      FANCM role in stabilizing ICL-stalled replication forks, we present evidence that 
      Mph1 prevents ICL-stalled replication forks from collapsing into double-strand 
      breaks. This unique repair function of Mph1 is specific for ICL damage and does 
      not extend to other types of damage. These studies reveal the functional 
      conservation of the FA pathway and validate the yeast model for future studies to 
      further elucidate the mechanism of the FA pathway.
FAU - Daee, Danielle L
AU  - Daee DL
AD  - Genome Instability Section, Genetics, and Molecular Biology Branch, National 
      Human Genome Research Institute, National Institutes of Health, Bethesda, 
      Maryland 20892, USA
FAU - Ferrari, Elisa
AU  - Ferrari E
FAU - Longerich, Simonne
AU  - Longerich S
FAU - Zheng, Xiao-feng
AU  - Zheng XF
FAU - Xue, Xiaoyu
AU  - Xue X
FAU - Branzei, Dana
AU  - Branzei D
FAU - Sung, Patrick
AU  - Sung P
FAU - Myung, Kyungjae
AU  - Myung K
LA  - eng
GR  - 242928/ERC_/European Research Council/International
GR  - Z01 HG012003/ImNIH/Intramural NIH HHS/United States
GR  - HG012003-09/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120612
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.6.1.- (FANCM protein, human)
RN  - EC 3.6.1.- (MPH1 protein, S cerevisiae)
RN  - EC 3.6.1.- (RAD5 protein, S cerevisiae)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - DEAD-box RNA Helicases/genetics/*physiology
MH  - DNA Helicases/genetics/*physiology
MH  - DNA Repair/*physiology
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Flow Cytometry
MH  - Humans
MH  - Mutation
MH  - Saccharomyces cerevisiae/*genetics
MH  - Saccharomyces cerevisiae Proteins/genetics/*physiology
PMC - PMC3410997
EDAT- 2012/06/15 06:00
MHDA- 2012/11/06 06:00
PMCR- 2013/08/03
CRDT- 2012/06/15 06:00
PHST- 2012/06/15 06:00 [entrez]
PHST- 2012/06/15 06:00 [pubmed]
PHST- 2012/11/06 06:00 [medline]
PHST- 2013/08/03 00:00 [pmc-release]
AID - S0021-9258(20)47853-6 [pii]
AID - M112.369918 [pii]
AID - 10.1074/jbc.M112.369918 [doi]
PST - ppublish
SO  - J Biol Chem. 2012 Aug 3;287(32):26563-75. doi: 10.1074/jbc.M112.369918. Epub 2012 
      Jun 12.