PMID- 22773384 OWN - NLM STAT- MEDLINE DCOM- 20121031 LR - 20211021 IS - 1528-0020 (Electronic) IS - 0006-4971 (Print) IS - 0006-4971 (Linking) VI - 120 IP - 8 DP - 2012 Aug 23 TI - Full reconstitution of human platelets in humanized mice after macrophage depletion. PG - 1713-6 LID - 10.1182/blood-2012-01-407890 [doi] AB - Cotransplantation of human fetal thymic tissue and CD34(+) fetal liver cells in nonobese diabetic (NOD)/severe combined immunodeficiency (SCID) or NOD/SCID/gammac(-/-) mice results in the development of multilineage human hematopoietic cells. In this study, we show that these humanized mice had extremely low levels of human platelets. The presence of human megakaryocytes at a normal concentration in the bone marrow suggests that human megakaryocytic differentiation occurred efficiently in these mice. Rapid increase in human platelets in blood to levels comparable with those of human peripheral blood mononuclear cells (PBMCs) after macrophage depletion indicates that mouse macrophages are responsible for the poor human platelet reconstitution in humanized mice. In support of this possibility, human platelets were rapidly rejected after infusion into untreated mice, but persisted in macrophage-depleted mice. These findings indicate that inhibition or depletion of recipient mouse macrophages may provide a useful means for evaluating human thrombopoiesis and platelet function in vivo using immunodeficient mice. FAU - Hu, Zheng AU - Hu Z AD - Columbia Center for Translational Immunology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA. FAU - Yang, Yong-Guang AU - Yang YG LA - eng GR - RC1 HL100117/HL/NHLBI NIH HHS/United States GR - P01 AI045897/AI/NIAID NIH HHS/United States GR - R01 AI064569/AI/NIAID NIH HHS/United States GR - P01AI045897/AI/NIAID NIH HHS/United States GR - RC1HL100117/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20120706 PL - United States TA - Blood JT - Blood JID - 7603509 RN - 0 (Antigens, CD34) SB - IM MH - Animals MH - Antigens, CD34/immunology MH - Blood Cell Count MH - Blood Platelets/*cytology/immunology MH - *Hematopoietic Stem Cell Transplantation MH - Hematopoietic Stem Cells/immunology MH - Humans MH - Leukocytes, Mononuclear/cytology MH - Liver/cytology MH - Macrophages/*cytology MH - Megakaryocytes/cytology MH - Mice MH - Mice, Inbred NOD MH - Mice, SCID MH - Platelet Count MH - *Thrombopoiesis MH - Thymus Gland/*transplantation MH - Transplantation, Heterologous PMC - PMC3429310 EDAT- 2012/07/10 06:00 MHDA- 2012/11/01 06:00 PMCR- 2013/08/23 CRDT- 2012/07/10 06:00 PHST- 2012/07/10 06:00 [entrez] PHST- 2012/07/10 06:00 [pubmed] PHST- 2012/11/01 06:00 [medline] PHST- 2013/08/23 00:00 [pmc-release] AID - S0006-4971(20)49110-4 [pii] AID - 2012/407890 [pii] AID - 10.1182/blood-2012-01-407890 [doi] PST - ppublish SO - Blood. 2012 Aug 23;120(8):1713-6. doi: 10.1182/blood-2012-01-407890. Epub 2012 Jul 6.