PMID- 23000963
OWN - NLM
STAT- MEDLINE
DCOM- 20130111
LR  - 20220318
IS  - 1476-4679 (Electronic)
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 14
IP  - 10
DP  - 2012 Oct
TI  - Dll1+ secretory progenitor cells revert to stem cells upon crypt damage.
PG  - 1099-1104
LID - 10.1038/ncb2581 [doi]
AB  - Lgr5+ intestinal stem cells generate enterocytes and secretory cells. Secretory 
      lineage commitment requires Notch silencing. The Notch ligand Dll1 is expressed 
      by a subset of immediate stem cell daughters. Lineage tracing in 
      Dll1(GFP-ires-CreERT2) knock-in mice reveals that single Dll1(high) cells 
      generate small, short-lived clones containing all four secretory cell types. 
      Lineage specification thus occurs in immediate stem cell daughters through Notch 
      lateral inhibition. Cultured Dll1(high) cells form long-lived organoids 
      (mini-guts) on brief Wnt3A exposure. When Dll1(high) cells are genetically marked 
      before tissue damage, stem cell tracing events occur. Thus, secretory progenitors 
      exhibit plasticity by regaining stemness on damage.
FAU - van Es, Johan H
AU  - van Es JH
AD  - Hubrecht Institute for Developmental Biology and Stem Cell Research & University 
      Medical Centre Utrecht, Uppsalalaan 8, 3584CT Utrecht, Netherlands.
FAU - Sato, Toshiro
AU  - Sato T
AD  - Hubrecht Institute for Developmental Biology and Stem Cell Research & University 
      Medical Centre Utrecht, Uppsalalaan 8, 3584CT Utrecht, Netherlands.
FAU - van de Wetering, Marc
AU  - van de Wetering M
AD  - Hubrecht Institute for Developmental Biology and Stem Cell Research & University 
      Medical Centre Utrecht, Uppsalalaan 8, 3584CT Utrecht, Netherlands.
FAU - Lyubimova, Anna
AU  - Lyubimova A
AD  - Dept. of Physics & Biology, Massachusetts Institute of Technology, 77 
      Massachusetts Avenue, Cambridge, MA 02139, USA.
FAU - Yee Nee, Annie Ng
AU  - Yee Nee AN
AD  - Institute of Medical Biology, 06-06 Immunos, Singapore.
FAU - Gregorieff, Alex
AU  - Gregorieff A
AD  - Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, 
      Toronto, Ontario M5G 1X5, Canada.
FAU - Sasaki, Nobuo
AU  - Sasaki N
AD  - Hubrecht Institute for Developmental Biology and Stem Cell Research & University 
      Medical Centre Utrecht, Uppsalalaan 8, 3584CT Utrecht, Netherlands.
FAU - Zeinstra, Laura
AU  - Zeinstra L
AD  - Hubrecht Institute for Developmental Biology and Stem Cell Research & University 
      Medical Centre Utrecht, Uppsalalaan 8, 3584CT Utrecht, Netherlands.
FAU - van den Born, Maaike
AU  - van den Born M
AD  - Hubrecht Institute for Developmental Biology and Stem Cell Research & University 
      Medical Centre Utrecht, Uppsalalaan 8, 3584CT Utrecht, Netherlands.
FAU - Korving, Jeroen
AU  - Korving J
AD  - Hubrecht Institute for Developmental Biology and Stem Cell Research & University 
      Medical Centre Utrecht, Uppsalalaan 8, 3584CT Utrecht, Netherlands.
FAU - Martens, Anton C M
AU  - Martens ACM
AD  - UMC Utrecht, Dept. of Immunology and Cell Biology, PO BOX 85090, 3508AB Utrecht, 
      Netherlands.
FAU - Barker, Nick
AU  - Barker N
AD  - Institute of Medical Biology, 06-06 Immunos, Singapore.
FAU - van Oudenaarden, Alexander
AU  - van Oudenaarden A
AD  - Dept. of Physics & Biology, Massachusetts Institute of Technology, 77 
      Massachusetts Avenue, Cambridge, MA 02139, USA.
FAU - Clevers, Hans
AU  - Clevers H
AD  - Hubrecht Institute for Developmental Biology and Stem Cell Research & University 
      Medical Centre Utrecht, Uppsalalaan 8, 3584CT Utrecht, Netherlands.
LA  - eng
GR  - U54 CA143874/CA/NCI NIH HHS/United States
GR  - U54CA143874/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20120923
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Dlk1 protein, mouse)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Receptors, Notch)
RN  - 0 (Wnt3A Protein)
RN  - 0 (Wnt3a protein, mouse)
SB  - IM
MH  - Animals
MH  - Calcium-Binding Proteins
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Gene Knock-In Techniques
MH  - Intercellular Signaling Peptides and Proteins/analysis/genetics/*metabolism
MH  - Intestinal Mucosa/*metabolism
MH  - Mice
MH  - Organoids/metabolism
MH  - Receptors, Notch/antagonists & inhibitors/metabolism
MH  - Stem Cells/*metabolism
MH  - Wnt3A Protein/pharmacology
PMC - PMC3789123
MID - NIHMS506945
EDAT- 2012/09/25 06:00
MHDA- 2013/01/12 06:00
PMCR- 2013/10/03
CRDT- 2012/09/25 06:00
PHST- 2012/05/23 00:00 [received]
PHST- 2012/08/16 00:00 [accepted]
PHST- 2012/09/25 06:00 [entrez]
PHST- 2012/09/25 06:00 [pubmed]
PHST- 2013/01/12 06:00 [medline]
PHST- 2013/10/03 00:00 [pmc-release]
AID - 10.1038/ncb2581 [doi]
PST - ppublish
SO  - Nat Cell Biol. 2012 Oct;14(10):1099-1104. doi: 10.1038/ncb2581. Epub 2012 Sep 23.