PMID- 23071634
OWN - NLM
STAT- MEDLINE
DCOM- 20130402
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 10
DP  - 2012
TI  - Comparison of hematopoietic stem cells derived from fresh and cryopreserved whole 
      cord blood in the generation of humanized mice.
PG  - e46772
LID - 10.1371/journal.pone.0046772 [doi]
LID - e46772
AB  - To study the function and maturation of the human hematopoietic and immune system 
      without endangering individuals, translational human-like animal models are 
      needed. We compare the efficiency of CD34(+) stem cells isolated from 
      cryopreserved cord blood from a blood bank (CCB) and fresh cord blood (FCB) in 
      generating highly engrafted humanized mice in NOD-SCID IL2Rgamma(null) (NSG) rodents. 
      Interestingly, the isolation of CD34(+) cells from CCB results in a lower yield 
      and purity compared to FCB. The purity of CD34(+) isolation from CCB decreases 
      with an increasing number of mononuclear cells that is not evident in FCB. 
      Despite the lower yield and purity of CD34(+) stem cell isolation from CCB 
      compared to FCB, the overall reconstitution with human immune cells (CD45) and 
      the differentiation of its subpopulations e.g., B cells, T cells or monocytes is 
      comparable between both sources. In addition, independent of the cord blood 
      origin, human B cells are able to produce high amounts of human IgM antibodies 
      and human T cells are able to proliferate after stimulation with anti-CD3 
      antibodies. Nevertheless, T cells generated from FCB showed increased response to 
      restimulation with anti-CD3. Our study reveals that the application of CCB 
      samples for the engraftment of humanized mice does not result in less engraftment 
      or a loss of differentiation and function of its subpopulations. Therefore, CCB 
      is a reasonable alternative to FCB and allows the selection of specific genotypes 
      (or any other criteria), which allows scientists to be independent from the daily 
      changing birth rate.
FAU - Scholbach, Johanna
AU  - Scholbach J
AD  - Institute of Clinical Immunology, University of Leipzig, Leipzig, Germany.
FAU - Schulz, Anett
AU  - Schulz A
FAU - Westphal, Florian
AU  - Westphal F
FAU - Egger, Dietmar
AU  - Egger D
FAU - Wege, Anja Kathrin
AU  - Wege AK
FAU - Patties, Ina
AU  - Patties I
FAU - Koberle, Margarethe
AU  - Koberle M
FAU - Sack, Ulrich
AU  - Sack U
FAU - Lange, Franziska
AU  - Lange F
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20121011
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, CD34)
RN  - 0 (Immunoglobulins)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - *Blood Preservation
MH  - Cell Proliferation
MH  - Cell Separation
MH  - *Cryopreservation
MH  - *Fetal Blood
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/metabolism/*physiology
MH  - Humans
MH  - Immunoglobulins/blood
MH  - Lymphocytes/metabolism/physiology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Mice, Transgenic
PMC - PMC3469562
COIS- Competing Interests: The authors have read the journal's policy and have the 
      following conflicts: one of the authors (DE) is working at Vita34 AG (Leipzig, 
      Germany). This company provided the cryopreserved cord blood samples. This does 
      not alter the authors' adherence to all the PLOS ONE policies on sharing data and 
      materials.
EDAT- 2012/10/17 06:00
MHDA- 2013/04/03 06:00
PMCR- 2012/10/11
CRDT- 2012/10/17 06:00
PHST- 2012/05/11 00:00 [received]
PHST- 2012/09/05 00:00 [accepted]
PHST- 2012/10/17 06:00 [entrez]
PHST- 2012/10/17 06:00 [pubmed]
PHST- 2013/04/03 06:00 [medline]
PHST- 2012/10/11 00:00 [pmc-release]
AID - PONE-D-12-13781 [pii]
AID - 10.1371/journal.pone.0046772 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(10):e46772. doi: 10.1371/journal.pone.0046772. Epub 2012 Oct 11.