PMID- 23152935
OWN - NLM
STAT- MEDLINE
DCOM- 20130416
LR  - 20211021
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 2
DP  - 2012
TI  - Quantitative, spectrally-resolved intraoperative fluorescence imaging.
PG  - 798
LID - 10.1038/srep00798 [doi]
LID - 798
AB  - Intraoperative visual fluorescence imaging (vFI) has emerged as a promising aid 
      to surgical guidance, but does not fully exploit the potential of the fluorescent 
      agents that are currently available. Here, we introduce a quantitative 
      fluorescence imaging (qFI) approach that converts spectrally-resolved data into 
      images of absolute fluorophore concentration pixel-by-pixel across the surgical 
      field of view (FOV). The resulting estimates are linear, accurate, and precise 
      relative to true values, and spectral decomposition of multiple fluorophores is 
      also achieved. Experiments with protoporphyrin IX in a glioma rodent model 
      demonstrate in vivo quantitative and spectrally-resolved fluorescence imaging of 
      infiltrating tumor margins for the first time. Moreover, we present images from 
      human surgery which detect residual tumor not evident with state-of-the-art vFI. 
      The wide-field qFI technique has broad implications for intraoperative surgical 
      guidance because it provides near real-time quantitative assessment of multiple 
      fluorescent biomarkers across the operative field.
FAU - Valdes, Pablo A
AU  - Valdes PA
AD  - Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA. 
      Pablo.A.Valdes@Dartmouth.edu
FAU - Leblond, Frederic
AU  - Leblond F
FAU - Jacobs, Valerie L
AU  - Jacobs VL
FAU - Wilson, Brian C
AU  - Wilson BC
FAU - Paulsen, Keith D
AU  - Paulsen KD
FAU - Roberts, David W
AU  - Roberts DW
LA  - eng
GR  - R01NS052274-01A2/NS/NINDS NIH HHS/United States
GR  - K25 CA138578/CA/NCI NIH HHS/United States
GR  - P30 CA023108/CA/NCI NIH HHS/United States
GR  - R01 NS052274/NS/NINDS NIH HHS/United States
GR  - K25CA138578/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20121112
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Protoporphyrins)
RN  - C2K325S808 (protoporphyrin IX)
SB  - IM
MH  - Animals
MH  - Brain Neoplasms/diagnosis/*surgery
MH  - *Fluorescence
MH  - Fluorescent Dyes
MH  - Glioma/diagnosis/surgery
MH  - Humans
MH  - Intraoperative Period
MH  - Optical Imaging/instrumentation/*methods
MH  - Phantoms, Imaging
MH  - Protoporphyrins
MH  - Rats
PMC - PMC3497712
COIS- The authors declare a US provisional patent application serial No. 61/588,708 on 
      some aspects of the technology used in this study.
EDAT- 2012/11/16 06:00
MHDA- 2013/04/17 06:00
PMCR- 2012/11/12
CRDT- 2012/11/16 06:00
PHST- 2012/09/11 00:00 [received]
PHST- 2012/10/01 00:00 [accepted]
PHST- 2012/11/16 06:00 [entrez]
PHST- 2012/11/16 06:00 [pubmed]
PHST- 2013/04/17 06:00 [medline]
PHST- 2012/11/12 00:00 [pmc-release]
AID - srep00798 [pii]
AID - 10.1038/srep00798 [doi]
PST - ppublish
SO  - Sci Rep. 2012;2:798. doi: 10.1038/srep00798. Epub 2012 Nov 12.